Nanosilver-loaded PMMA bone cement doped with different bioactive glasses – evaluation of cytocompatibility, antibacterial activity, and mechanical properties
- 17 February 2021
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Biomaterials Science
- Vol. 9 (8), 3112-3126
- https://doi.org/10.1039/d1bm00079a
Abstract
Nanosilver-loaded PMMA bone cement (BC-AgNp) is a novel cement developed as a replacement for conventional cements. Despite favorable properties and antibacterial activity, BC-AgNp still lacks biodegradability and bioactivity. Hence, we investigated the doping with bioactive glasses (BGs) to create a new bioactive BC characterized by time-varying porosity and gradual release of AgNp. The BC Cemex was used as the base material and modified simultaneously with the AgNp and BGs: melted 45S5 and 13-93B3 glasses with various particles size and sol-gel derived SiO2/CaO microparticles. The effect of BGs addition was examined by microscopic analysis, an assessment of setting parameters, wettability, FTIR and UV-VIS spectroscopy, mechanical testing, hemo- and cytocompatibility and antibacterial efficiency studies. The results show that it is possible to incorporate various BGs into BC-AgNp that lead to different properties depending on the type and size of BGs. The smaller particles of melted BGs showed higher porosity and better antibacterial properties with the moderate deterioration of mechanical properties. The sol-gel derived BGs, however, displayed a tendency for agglomeration and random distribution in BC-AgNp. The BGs with greater solubility more efficiently improves the antibacterial properties of BC-AgNp. Besides, the unreacted MMA monomer release could negatively influence the cellular response. Despite that, the cements doped with different BGs are suitable for medical applications.Keywords
Funding Information
- Narodowe Centrum Nauki (2016/21/B/NZ5/00217)
- Gdański Uniwersytet Medyczny (ST-57)
- Narodowa Agencja Wymiany Akademickiej (1.PROM2.2019)
- Horizon 2020 Framework Programme (739566)
This publication has 81 references indexed in Scilit:
- Antibiofilm Properties of Silver and Gold Incorporated PU, PCLm, PC and PMMA Nanocomposites under Two Shear ConditionsPLOS ONE, 2013
- Molecular interactions in coupled PMMA–bioglass hybrid networksJournal of Materials Chemistry B, 2012
- Physical characterization and osteogenic activity of the quaternized chitosan-loaded PMMA bone cementActa Biomaterialia, 2012
- A facile fabrication of porous PMMA as a potential bone substituteMaterials Science and Engineering: C, 2011
- Cytotoxicity of 45S5 bioglass paste used for dentine hypersensitivity treatmentJournal of Dentistry, 2011
- Influence of lactose addition to gentamicin-loaded acrylic bone cement on the kinetics of release of the antibiotic and the cement propertiesActa Biomaterialia, 2010
- Pore distribution and material properties of bone cement cured at different temperaturesActa Biomaterialia, 2010
- Methotrexate released in vitro from bone cement inhibits human stem cell proliferation in S/G2 phaseInternational Orthopaedics, 2009
- Synthesis and in vitro bioactivity of a borate-based bioglassMaterials Letters, 2007
- An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experimentsJournal of Materials Research, 1992