Don't let go: co‐fractionation mass spectrometry for untargeted mapping of protein–metabolite interactomes
Open Access
- 5 February 2023
- journal article
- review article
- Published by Wiley in The Plant Journal
- Vol. 113 (5), 904-914
- https://doi.org/10.1111/tpj.16084
Abstract
The chemical complexity of metabolomes goes hand in hand with their functional diversity. Small molecules have many essential roles, many of which are executed by binding and modulating the function of a protein partner. The complex and dynamic protein-metabolite interaction (PMI) network underlies most if not all biological processes, but remains under-characterized. Herein, we highlight how co-fractionation mass spectrometry (CF-MS), a well-established approach to map protein assemblies, can be used for proteome and metabolome identification of the PMIs. We will review recent CF-MS studies, discuss the main advantages and limitations, summarize the available CF-MS guidelines, and outline future challenges and opportunities.Keywords
Funding Information
- National Science Foundation (2226270)
This publication has 100 references indexed in Scilit:
- A Census of Human Soluble Protein ComplexesCell, 2012
- The Arabidopsis NPR1 Protein Is a Receptor for the Plant Defense Hormone Salicylic AcidCell Reports, 2012
- NPR3 and NPR4 are receptors for the immune signal salicylic acid in plantsNature, 2012
- High-pH reversed-phase chromatography with fraction concatenation for 2D proteomic analysisExpert Review of Proteomics, 2012
- Screening Natural Products for Inhibitors of Quinone Reductase-2 Using Ultrafiltration LC−MSAnalytical Chemistry, 2010
- Extensive In Vivo Metabolite-Protein Interactions Revealed by Large-Scale Systematic AnalysesCell, 2010
- Megadalton Complexes in the Chloroplast Stroma of Arabidopsis thaliana Characterized by Size Exclusion Chromatography, Mass Spectrometry, and Hierarchical ClusteringMolecular & Cellular Proteomics, 2010
- Metabolomics for Functional Genomics, Systems Biology, and BiotechnologyAnnual Review of Plant Biology, 2010
- STITCH 2: an interaction network database for small molecules and proteinsNucleic Acids Research, 2009
- Whence cometh the allosterome?Proceedings of the National Academy of Sciences of the United States of America, 2006