Mechanistic Insight into Royal Protein Inhibiting the Gram-Positive Bacteria
Open Access
- 6 January 2021
- journal article
- research article
- Published by MDPI AG in Biomolecules
- Vol. 11 (1), 64
- https://doi.org/10.3390/biom11010064
Abstract
Royal jelly (RJ), a natural honeybee product, has a wide range of antibacterial activities. N-glycosylated major royal jelly protein 2 (N-MRJP2), purified from RJ, can inhibit the growth of Paenibacillus larvae (P. larvae, Gram-positive), a contagious etiological agent of the American foulbrood disease of honeybees. However, the inhibitory mechanism is largely unknown. Antibacterial assay and membrane proteome were conducted to investigate the inhibition capacity of RJ from different instar larvae and P. larvae treated by N-MRJP2, respectively. The similar antibacterial efficiency of RJ from different larval instar indicates that RJ is vital for the adaptive immune defense of small larvae. The killing of P. larvae by N-MRJP2 is achieved by disturbing the cell wall biosynthesis, increasing the permeability of cell membrane, hindering aerobic respiration, restraining cell division and inducing cell death. This demonstrates that RJ is critical for the passive immunity of immature larvae and N-MRJP2 can be used as natural antibiotic substance to resist P. larvae, even for other gram-positive bacteria. This constitutes solid evidence that RJ and N-MRJP2 have potentials as novel antibacterial agents.Funding Information
- Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2015-IAR)
This publication has 73 references indexed in Scilit:
- Mechanism of Action of Recombinant Acc-Royalisin from Royal Jelly of Asian Honeybee against Gram-Positive BacteriaPLOS ONE, 2012
- Selective Penicillin-Binding Protein Imaging Probes Reveal Substructure in Bacterial Cell DivisionACS Chemical Biology, 2012
- Identification and Functional Analysis of the S-Layer Protein SplA of Paenibacillus larvae, the Causative Agent of American Foulbrood of Honey BeesPLoS Pathogens, 2012
- Effect of Royal Jelly on spatial learning and memory in rat model of streptozotocin-induced sporadic Alzheimer′s diseaseIbnosina Journal of Medicine and Biomedical Sciences, 2012
- Hypoxia Triggers AMPK Activation through Reactive Oxygen Species-Mediated Activation of Calcium Release-Activated Calcium ChannelsMolecular and Cellular Biology, 2011
- Royal Jelly Facilitates Restoration of the Cognitive Ability in Trimethyltin-Intoxicated MiceEvidence-Based Complementary and Alternative Medicine, 2011
- PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotesBioinformatics, 2010
- Negative Correlation between Individual-Insect-Level Virulence and Colony-Level Virulence of Paenibacillus larvae , the Etiological Agent of American Foulbrood of HoneybeesApplied and Environmental Microbiology, 2009
- Evolution and mechanisms of long life and high fertility in queen honey beesAGE, 2008
- Immune pathways and defence mechanisms in honey bees Apis melliferaInsect Molecular Biology, 2006