Sleep- and time of day-linked RNA transcript expression in wild-type and IL1 receptor accessory protein-null mice
- 22 April 2020
- journal article
- research article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 128 (6), 1506-1522
- https://doi.org/10.1152/japplphysiol.00839.2019
Abstract
Sleep regulation involves interleukin-1 beta (IL1) family members, TNF, and circadian clock genes. Previously, we characterized spontaneous sleep and sleep after 8 h of sleep deprivation (SD) ending at zeitgeber time (ZT)4 and ZT16 in wild-type (WT) and IL1 receptor accessory protein (AcP)- and brain-specific AcP (AcPb)-knockout (KO) mice. Here, we applied quantitative reverse transcriptase polymerase chain reaction and Spearman gene pair expression correlation methods to characterize IL1, IL1 receptor 1 (IL1R1), AcP, AcPb, Period 1 (Per1), Clock, adenosine deaminase (Ada), peptidoglycan recognition protein 1 (Pglyrp1), and TNF mRNA expressions under conditions with distinct sleep phenotypes. In WT mice, IL1, IL1R1, AcP, Ada, and Clock mRNAs were higher at ZT4 (mid-sleep period) than at ZT16. mRNA expressions differed substantially in AcP and AcPb KO mice at those times. After SD ending at ZT4, only WT mice had a non-rapid eye movement sleep (NREMS) rebound, and AcPb and IL1R1 mRNA increases were unique to WT mice. In AcPb KO mice, which have spontaneous high EEG slow wave power, AcP and Pglyrp1 mRNAs were elevated relative to WT mice at ZT4. At ZT4, the AcPb KO - WT Spearman correlation difference networks showed high positive correlations between IL1R1 and IL1, Per1, and Clock and high negative correlations between TNF and Pglyrp1 and Ada. At ZT16, the WT mice gene pair expression network was mostly negative, whereas in AcP KO mice, which have substantially more rapid eye movement sleep than WT mice, it was all positive. We conclude that gene pair expression correlations depend on the presence of AcP and AcPb. NEW & NOTEWORTHY Spearman gene pair expression correlations depend upon the presence or absence of interleukin-1 receptor accessory protein and upon sleep phenotype.Funding Information
- NIH NINDS (NS025378)
- National Science Foundation (1553067)
This publication has 76 references indexed in Scilit:
- Involvement of cytokines in slow wave sleepProgress in Brain Research, 2011
- Local sleep in awake ratsNature, 2011
- Whisker stimulation increases expression of nerve growth factor- and interleukin-1β-immunoreactivity in the rat somatosensory cortexBrain Research, 2010
- How (and why) the immune system makes us sleepNature Reviews Neuroscience, 2009
- Sleep as a fundamental property of neuronal assembliesNature Reviews Neuroscience, 2008
- Tumor necrosis factor α: Activity dependent expression and promotion of cortical column sleep in ratsNeuroscience, 2008
- Cytokine mRNA induction by interleukin-1β or tumor necrosis factor α in vitro and in vivoBrain Research, 2008
- TNF-α suppresses the expression of clock genes by interfering with E-box-mediated transcriptionProceedings of the National Academy of Sciences of the United States of America, 2007
- TNFα siRNA reduces brain TNF and EEG delta wave activity in ratsBrain Research, 2007
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001