First identification and genomic characterization of equine hepacivirus sub-type 3 strain in China
- 1 December 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Virus Genes
- Vol. 56 (6), 777-780
- https://doi.org/10.1007/s11262-020-01792-y
Abstract
Equine Hepacivirus(EqHV) is a newly discovered equine virus that is classified under theHepacivirusgenus of theFlaviviridaefamily. There are three sub-types of EqHV worldwide namely; sub-types 1-3. The majority of EqHV sub-type 1 strains were found in China. While different sub-types have been found in Japan and USA, therefore, to investigate whether the other sub-types of EqHV strains were present in China, a total of 60 horse serum samples were collected and screened for EqHV RNA through RT-PCR. The results revealed that 19 serum samples were RNA-positive (19/60) and the EqHV detection rate was 31.67%. One EqHV strain named GD23 was obtained and its near-complete genome sequence was acquired. Analysis of nucleotidep-distance with reference to the entire polyprotein gene revealed that GD23 was classified into sub-type 3. In addition, the phylogenetic analysis demonstrated that GD23 was clustered together with EqHV strains of sub-type 3 in other countries. The present study is the first to identify an EqHV sub-type 3 strain in China.Funding Information
- The National Natural Science Foundation of China (31372448, 31672563)
- the Special Fund for Agro-scientific Research in the Public Interest (201303042)
- the national key Research and Development Program of China (2016YFD0501004)
- the national key Research and Development Program of China (2016YFD0501010)
- the Promote Scientific and Technological Development Program (2013B040200032)
- The Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases (2013A061401013)
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