Comparative analyses of the Sox9a-Amh-Cyp19a1a regulatory Cascade in Autotetraploid fish and its diploid parent
Open Access
- 21 March 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Genetics
- Vol. 21 (1), 1-9
- https://doi.org/10.1186/s12863-020-00840-8
Abstract
Autotetraploid Carassius auratus (4nRCC, 4n = 200, RRRR) was derived from the whole genome duplication of diploid red crucian carp (Carassius auratus red var.) (2nRCC, 2n = 100, RR). To investigate the genetic effects of tetraploidization, we analyzed DNA variation, epigenetic modification and gene expression changes in the Sox9a-Amh-Cyp19a1a regulatory cascade between 4nRCC and 2nRCC. We found that the Sox9a gene contained two variants in 2nRCC and four variants in 4nRCC. Compared with that in 2nRCC, DNA methylation in the promoter regions of the Amh and Cyp19a1a genes in 4nRCC was altered by single nucleotide polymorphism (SNP) mutations, which resulted in the insertions and deletions of CpG sites, and the methylation levels of the Sox9a, Amh and Cyp19a1a genes increased after tetraploidization. The gene expression level of the Sox9a-Amh-Cyp19a1a regulatory cascade was downregulated in 4nRCC compared with that in 2nRCC. The above results demonstrate that tetraploidization leads to significant changes in the genome, epigenetic modification and gene expression in the Sox9a-Amh-Cyp19a1a regulatory cascade; these findings increase the extant knowledge regarding the effects of polyploidization.Keywords
Funding Information
- National Natural Science Foundation of China (31430088, 31730098)
- Earmarked Fund for China Agriculture Research System (CARS-45)
- Hunan Provincial Science and Technology Department (2017NK1031)
- Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486)
- Key Research and Development Program of Hunan Province (2018NK2072)
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