Haspin Modulates the G2/M Transition Delay in Response to Polarization Failures in Budding Yeast
Open Access
- 28 January 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
Symmetry breaking by cellular polarization is an exquisite requirement for the cell-cycle of Saccharomyces cerevisiae cells, as it allows bud emergence and growth. This process is based on the formation of polarity clusters at the incipient bud site, first, and the bud tip later in the cell-cycle, that overall promote bud emission and growth. Given the extreme relevance of this process, a surveillance mechanism, known as the morphogenesis checkpoint, has evolved to coordinate the formation of the bud and cell cycle progression, delaying mitosis in the presence of morphogenetic problems. The atypical protein kinase haspin is responsible for histone H3-T3 phosphorylation and, in yeast, for resolution of polarity clusters in mitosis. Here, we report a novel role for haspin in the regulation of the morphogenesis checkpoint in response to polarity insults. Particularly, we show that cells lacking the haspin ortholog Alk1 fail to achieve sustained checkpoint activation and enter mitosis even in the absence of a bud. In alk1Δ cells, we report a reduced phosphorylation of Cdc28-Y19, which stems from a premature activation of the Mih1 phosphatase. Overall, the data presented in this work define yeast haspin as a novel regulator of the morphogenesis checkpoint in Saccharomyces cerevisiae, where it monitors polarity establishment and it couples bud emergence to the G2/M cell cycle transition.Funding Information
- Associazione Italiana per la Ricerca sul Cancro (17919, 21806)
- Ministero dell’Istruzione, dell’Università e della Ricerca
This publication has 79 references indexed in Scilit:
- Feedback control of Swe1p degradation in the yeast morphogenesis checkpointMolecular Biology of the Cell, 2013
- Fiji: an open-source platform for biological-image analysisNature Methods, 2012
- A link between mitotic entry and membrane growth suggests a novel model for cell size controlThe Journal of cell biology, 2012
- A Positive Feedback Loop Involving Haspin and Aurora B Promotes CPC Accumulation at Centromeres in MitosisCurrent Biology, 2011
- The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphataseMolecular Biology of the Cell, 2011
- Molecular Dissection of the Checkpoint Kinase Hsl1pMolecular Biology of the Cell, 2009
- The Rho GDI Rdi1 Regulates Rho GTPases by Distinct MechanismsMolecular Biology of the Cell, 2008
- Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1The Journal of cell biology, 2008
- The budding yeast PP2ACdc55 protein phosphatase prevents the onset of anaphase in response to morphogenetic defectsThe Journal of cell biology, 2007
- Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosisNature, 1989