Unraveling Cell-Type-Specific Targeted Delivery of Membrane-Camouflaged Nanoparticles with Plasmonic Imaging
- 8 June 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 20 (7), 5228-5235
- https://doi.org/10.1021/acs.nanolett.0c01503
Abstract
Cell-membrane-camouflaged nanoparticles (CMC-NPs) have been increasingly exploited to develop various therapeutic tools due to their high biocompatibility and cell-type-specific tumor-targeting properties. However, the molecular mechanism of CMC-NPs for homotypic targeting remains elusive. Here, we develop a plasmonic imaging method by coating gold nanoparticles (AuNPs) with cancer cell membranes and perform plasmonic imaging of the interactions between CMC-NPs and living cells at the single-cell level. Quantitative analysis of CMC-NPs in a different clustering status reveals that the presence of cell membranes on CMC-NPs results in a 7-fold increase in homotypic cell delivery and nearly 2 orders of magnitude acceleration of the intracellular agglomeration process. Significantly, we identify that integrin αvβ3, a cell surface receptor abundantly expressed in tumor cells, is critical for the selective cell recognition of CMC-NPs. We thus establish a single-cell plasmonic imaging platform for probing NP–cell interactions, which sheds new light on the therapeutic applications of CMC-NPs.Keywords
Funding Information
- Ministry of Science and Technology of the People's Republic of China (2016YFA0400900)
- K. C. Wong Education Foundation
- National Natural Science Foundation of China (21834007, 21974089, 21991134, 31771102, 81773434, 91953016)
- Chinese Academy of Sciences (QYZDJ-SSW-SLH031)
- Major Science and Technology Innovation Program of Shanghai Municipal Education Commission (2019-01-07-00-01-E00059)
- LU Jiaxi International Team of the Chinese Academy of Sciences
- Innovative Research Team of High-Level Local Universities in Shanghai
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