FARKLI LOKASYONLARDA YABANİ BİTKİ TÜRLERİNDEN İZOLE EDİLEN BAKTERİLERİN TANISI VE AZOT FİKSE ETME, FOSFOR, POTASYUM VE KALSİYUM ÇÖZME ÖZELLİKLERİNİN BELİRLENMESİ
- 18 November 2020
- journal article
- Published by Journal of Agriculture in Journal of Agriculture
- Vol. 3 (2), 71-90
- https://doi.org/10.46876/ja.825647
Abstract
Tr en Bu çalışmada, farklı illerden alınan 23 sağlıklı bitki örneğinden yapılan izolasyon sonucunda 246 bakteri straini elde edilmiştir. Tütünde yapılan HR testi ile bakteri strainlerinin patojen olmadıkları belirlenmiştir. Strainler Mikrobial Tanı Sistemi kullanılarak yağ asit metil analizi ile Arthbacter (17), Brevibacillus (12), Bacillus (65), Lysinibacillus (3), Herbaspirillum (7), Kocuria (21), Paucimonas (8), Pseudomonas (36) , Virgibacillus (3), Microbacterium (11), Micrococcus (8), Erwinia (4), Stenotrophomonas (8), Nesterenkonia (1), Achromobacter (1), Curtobacterium (5), Rhodococcus (7), Enterobacter (2), Escherichia (1), Chryseobacterium (1), Xanthomonas (3), Acinetobacter (5), Rothia (1), Paenibacillus (1), Ochrobacterium (1), Pantoea (1), Sphingbacterium (5), Rhizobium (3), Grimontia (1), Aeromonas (1), Brevundimonas (1), Phyllobacterium (1) ve Staphylococcus (1) olarak belirlenmiştir. Elde edilen bakteri strainleri azot fiksasyonu, fosfat, potasyum ve kalsiyum çözücü özellikleri bakımından test edilmiştir. Bunlar arasında Herbaspirillum huttiense (SK4, SK49), Microbacterium esteraromaticum (SK19, SK39, SY48), Achromobacter xylosoxidans (SK50), Paucimonas lemoignei (SK56), Pantoea agglomerans (SY43), Pseudomonas putida biotype B (YS2, DT17), Pseudomonas syringae pv. syringae (EP19) ve Pseudomonas pseudoalcaligenes (SA20) olmak üzere 12 tane strainin bütün testlerde pozitif sonuç verdiği, diğer strainlerin test sonuçlarının ise değişkenlik gösterdiği belirlenmiştir. In this study, 246 bacterial strains were isolated from 23 healthy plant samples were taken from different provinces. Bacterial strains were tested for hypersensitive response (HR) on tobacco. HR test showed that bacterial strains were not pathogenic. The strains were identified by using the Microbial Identification System. According to the fatty acid methyl ester analysis results bacterial strains were determined as follow; Arthbacter (17), Brevibacillus (12), Bacillus (65), Lysinibacillus (3), Herbaspirillum (7), Kocuria (21), Paucimonas (8), Pseudomonas (36), Virgibacillus (3), Microbacterium (11), Micrococcus (8), Erwinia (4), Stenotrophomonas (8), Nesterenkonia (1), Achromobacter (1), Curtobacterium (5), Rhodococcus (7), Enterobacter (2), Escherichia (1), Chryseobacterium (1), Xanthomonas (3), Acinetobacter (5), Rothia (1), Paenibacillus (1), Ochrobacterium (1), Pantoea (1), Sphingbacterium (5), Rhizobium (3), Grimontia (1), Aeromonas (1) , Brevundimonas (1), Phyllobacterium (1) ve Staphylococcus (1). The obtained bacterial strains were tested for nitrogen fixing, phosphate, potassium and calcium solubilising properties. Herbaspirillum huttiense (SK4, SK49), Microbacterium esteraromaticum (SK19, SK39, SY48), Achromobacter xylosoxidans (SK50), Paucimonas lemoignei (SK56), Pantoea agglomerans (SY43) , Pseudomonas putida biotype B (YS2, DT17), Pseudomonas syringae pv. syringae (EP19) and Pseudomonas pseudoalcaligenes (SA20) strains were found to be positive in all tests and the test results of other strains were found to be variable.Keywords
Funding Information
- Iğdır Üniversitesi Bilimsel Araştırma Projeleri Birimi (2017-FBE-A26)
This publication has 36 references indexed in Scilit:
- Efficient soil microorganisms: A new dimension for sustainable agriculture and environmental developmentAgriculture, Ecosystems & Environment, 2011
- MOBILIZATION OF POTASSIUM FROM WASTE MICA BY PLANT GROWTH PROMOTING RHIZOBACTERIA AND ITS ASSIMILATION BY MAIZE (ZEA MAYS) AND WHEAT (TRITICUM AESTIVUML.): A HYDROPONICS STUDY UNDER PHYTOTRON GROWTH CHAMBERJournal of Plant Nutrition, 2010
- Endophytic bacteria in cacti seeds can improve the development of cactus seedlingsEnvironmental and Experimental Botany, 2009
- Phosphate-solubilizing bacteria as inoculants for agriculture: use of updated molecular techniques in their studyAgronomy for Sustainable Development, 2001
- Comparison of Nitrogen-fixing Bacterial Flora of Rice Rhizosphere in the Fields Treated Long-term with Agrochemicals and Non-agrochemicalsMicrobes and Environments, 2001
- Aspergillus aculeatus as a rock phosphate solubilizerSoil Biology and Biochemistry, 2000
- Stress induced phosphate solubilization in bacteria isolated from alkaline soilsFEMS Microbiology Letters, 2000
- Processes Governing Phosphorus Availability in Temperate SoilsJournal of Environmental Quality, 2000
- An efficient microbiological growth medium for screening phosphate solubilizing microorganismsFEMS Microbiology Letters, 1999
- Activity of phosphate-dissolving bacteria in Egyptian soilsPlant and Soil, 1969