The whole blood phagocytosis assay: a clinically relevant test of neutrophil function and dysfunction in community-acquired pneumonia
Open Access
- 8 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Research Notes
- Vol. 13 (1), 1-5
- https://doi.org/10.1186/s13104-020-05034-0
Abstract
To refine and validate a neutrophil function assay with clinical relevance for patients with community-acquired pneumonia (CAP). Two phase cross-sectional study to standardise and refine the assay in blood from healthy volunteers and test neutrophil phagocytic function in hospital patients with CAP. Phase one: Healthy adult volunteers (n = 30). Phase two: Critical care patients with severe CAP (n = 16), ward-level patients with moderate CAP (n = 15) and respiratory outpatients (no acute disease, n = 15). Our full standard operating procedure for the assay is provided. Patients with severe CAP had significantly decreased neutrophil function compared to moderate severity disease (median phagocytic index 2.8 vs. 18.0, p = 0.014). Moderate severity pneumonia neutrophil function was significantly higher than control samples (median 18.0 vs. 1.6, p = 0.015). There was no significant difference between critical care and control neutrophil function (median 2.8 vs. 1.6, p = 0.752). Our whole blood neutrophil assay is simple, reproducible and clinically relevant. Changes in neutrophil function measured in this pneumonia cohort is in agreement with previous studies. The assay has potential to be used to identify individuals for clinical trials of immunomodulatory therapies, to risk-stratify patients with pneumonia, and to refine our understanding of ‘normal’ neutrophil function in infection.Keywords
Other Versions
Funding Information
- Wellcome Trust (104936/Z/14/Z)
This publication has 10 references indexed in Scilit:
- Functional Analysis of Phagocyte Activity in Whole Blood from HIV/Tuberculosis-Infected Individuals Using a Novel Flow Cytometry-Based AssayFrontiers in Immunology, 2017
- Augmented Passive Immunotherapy with P4 Peptide Improves Phagocyte Activity in Severe SepsisShock, 2016
- Immunomodulatory adjuvant therapy in severe community-acquired pneumoniaExpert Review of Respiratory Medicine, 2014
- Contrasting Inflammatory Responses in Severe and Non-severe Community-acquired PneumoniaInflammation, 2014
- Sepsis-induced immunosuppression: from cellular dysfunctions to immunotherapyNature Reviews Immunology, 2013
- C5a-mediated neutrophil dysfunction is RhoA-dependent and predicts infection in critically ill patientsBlood, 2011
- International Study of the Prevalence and Outcomes of Infection in Intensive Care UnitsJAMA, 2009
- Granulocyte–Macrophage Colony-stimulating Factor to Reverse Sepsis-associated ImmunosuppressionAmerican Journal of Respiratory and Critical Care Medicine, 2009
- Intraphagosomal Measurement of the Magnitude and Duration of the Oxidative BurstTraffic, 2009
- Intraphagosomal measurement of the magnitude and duration of the oxidative burst.Traffic, 2009