Pooling of samples to optimize SARS-CoV-2 diagnosis by RT-qPCR: comparative analysis of two protocols
Open Access
- 16 October 2020
- journal article
- editorial
- Published by Springer Science and Business Media LLC in European Journal of Clinical Microbiology & Infectious Diseases
- Vol. 40 (4), 889-892
- https://doi.org/10.1007/s10096-020-04071-8
Abstract
This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B, reduced agreement (91%) was observed in relation to individual testing. Inconsistencies observed were related to RT-qPCR results with higher cycle thresholds. These results suggest that pooling of samples before RNA extraction is preferable in terms of diagnostic for SARS-CoV-2.Keywords
Funding Information
- Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (#20-2551-0000265-9)
- Hospital de Clínicas de Porto Alegre (#2020-0163)
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