Genome editing reveals fitness effects of a gene for sexual dichromatism in Sulawesian fishes
Open Access
- 1 March 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 12 (1), 1-13
- https://doi.org/10.1038/s41467-021-21697-0
Abstract
Sexual selection drives rapid phenotypic diversification of mating traits. However, we know little about the causative genes underlying divergence in sexually selected traits. Here, we investigate the genetic basis of male mating trait diversification in the medaka fishes (genus Oryzias) from Sulawesi, Indonesia. Using linkage mapping, transcriptome analysis, and genome editing, we identify csf1 as a causative gene for red pectoral fins that are unique to male Oryzias woworae. A cis-regulatory mutation enables androgen-induced expression of csf1 in male fins. csf1-knockout males have reduced red coloration and require longer for mating, suggesting that coloration can contribute to male reproductive success. Contrary to expectations, non-red males are more attractive to a predatory fish than are red males. Our results demonstrate that integrating genomics with genome editing enables us to identify causative genes underlying sexually selected traits and provides a new avenue for testing theories of sexual selection.Funding Information
- MEXT | Japan Society for the Promotion of Science (18K14769, 16K14792, 26291093, 19K16232, 16H06279, 16H06279, 16J05534)
- NIBB Collaborative Research Program (17-313) NIG-JOINT
This publication has 113 references indexed in Scilit:
- MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and UsabilityMolecular Biology and Evolution, 2013
- Estimating divergence times in large molecular phylogeniesProceedings of the National Academy of Sciences of the United States of America, 2012
- Fiji: an open-source platform for biological-image analysisNature Methods, 2012
- A statistical framework for SNP calling, mutation discovery, association mapping and population genetical parameter estimation from sequencing dataBioinformatics, 2011
- The variant call format and VCFtoolsBioinformatics, 2011
- Easyfig: a genome comparison visualizerBioinformatics, 2011
- Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell IdentitiesMolecular Cell, 2010
- edgeR: a Bioconductor package for differential expression analysis of digital gene expression dataBioinformatics, 2009
- The Sequence Alignment/Map format and SAMtoolsBioinformatics, 2009
- trimAl: a tool for automated alignment trimming in large-scale phylogenetic analysesBioinformatics, 2009