Effect of bisphenols on telomerase expression and activity in breast cancer cell lines
- 24 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Molecular Biology Reports
- Vol. 47 (5), 3541-3549
- https://doi.org/10.1007/s11033-020-05444-0
Abstract
Bisphenol A (BPA), a monomer of polycarbonates and resins, was shown to induce the expression of telomerase enzyme which has been associated with breast cancer development and progression. However, the effects of BPA analogues, bisphenol F (BPF) and bisphenol S (BPS) on telomere-linked pathway have not been evaluated. Herein, MCF-7 (estrogen receptor (ER)-positive) and MDA-MB-231 (ER-negative) cells were treated with BPA, BPF and BPS ± estrogen receptor inhibitor (ERI), for 24 and/or 48 h. RNA expression and enzymatic activity of telomerase were measured using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and telomeric repeat amplification protocol (TRAP); respectively. Relative telomere length (RTL) was also measured using quantitative PCR. After 24 h, the three bisphenols resulted in a 2–3 folds increase in expression and activity of telomerase in MCF-7 but not in MDA-MB-231 cells, and this increase was prevented upon co-treatment with ERI. The observed increase in the expression and activity of telomerase after 24 h of treatment with bisphenols was associated with differential and modest ER-dependent lengthening in RTL at 48 h. Our results show that telomerase potentially mediates the effects of the three bisphenols in ER-positive breast carcinoma. Hence, further investigation is warranted to elucidate the telomerase-linked pathways that could underlie bisphenol-related effects.Funding Information
- American University of Beirut Medical Practice Plan
- Conseil National de la Recherche Scientifique
This publication has 42 references indexed in Scilit:
- Serum bisphenol-A concentration and sex hormone levels in menFertility and Sterility, 2013
- Circulating serum xenoestrogens and mammographic breast densityBreast Cancer Research, 2013
- The Exposure of Breast Cancer Cells to Fulvestrant and Tamoxifen Modulates Cell Migration DifferentlyBioMed Research International, 2013
- Effect of bisphenol A on the EGFR-STAT3 pathway in MCF-7 breast cancer cellsMolecular Medicine Reports, 2011
- The Influence of Endocrine Disrupting Chemicals on the Proliferation of ER.ALPHA. Knockdown-Human Breast Cancer Cell Line MCF-7; New Attempts by RNAi TechnologyACTA HISTOCHEMICA ET CYTOCHEMICA, 2009
- Chapel Hill bisphenol A expert panel consensus statement: Integration of mechanisms, effects in animals and potential to impact human health at current levels of exposureReproductive Toxicology, 2007
- Bisphenol A from dental polycarbonate crown upregulates the expression of hTERTJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2004
- Effects of environmental estrogenic chemicals on AP1 mediated transcription with estrogen receptors α and βThe Journal of Steroid Biochemistry and Molecular Biology, 2004
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Specific Association of Human Telomerase Activity with Immortal Cells and CancerScience, 1994