Distribution and molecular characterization of rickettsiae in ticks in Harbin area of Northeastern China

Abstract

Tick-borne rickettsioses are world-spreading infectious zoonoses. Ticks serve as reservoirs and vectors for Rickettsia and play a key role in transmission of rickettsioses. Most of the Chinese rickettsiosis patients are reported from Northeastern China but the distribution of tick and tick-borne Rickettsia species in Northeastern China remain poorly studied. In this study, a total of 1,286 ticks were captured from the seven counties of Harbin, an area in Northeastern China, and the tick-borne Rickettsia species were identified by PCR and sequencing of rrs, gltA, groEL, ompA and 17-kDa antigen-encoding genes. Of the 5 identified tick species, Haemaphysalis longicornis and Ixodes persulcatus were the predominant tick species in the livestock and vegetation, respectively. Rickettsia raoultii and "Candidatus Rickettsia tarasevichiae" were the two detectable Rickettsia species in the ticks with a 28.8% positive rate but no rickettsiae were found in ticks of Haemaphysalis concinna. R. raoultii detected in 37.6% of the Dermacentor nuttalli, Dermacentor silvarum and H. longicornis ticks while "Ca. R. tarasevichiae" was only present in 22.8% of the I. persulcatus ticks. In particular, the positive rate of both R. raoultii and "Ca. R. tarasevichiae" in ticks from the livestock (40.7%) was significantly higher than that from the vegetation (19.5%). The results indicate that the tick and tick-borne Rickettsia species are diverse in different regions of Harbin due to geographic difference and the ticks from livestock may play a more important role in transmission of rickettsioses to human. Author summary Rickettsiosis is a tick-borne infectious disease of global importance. The disease has been prevailing in Northeastern China but the distribution of tick and tick-borne Rickettsia species from different areas of Northeastern China remain poorly studied. We collected a total of 1,286 ticks in the seven counties with different geographic environments of Harbin, an area of Northeastern China, and all the ticks were classified as Dermacentor nuttalli, Dermacentor silvarum, Haemaphysalis concinna, Haemaphysalis longicornis or Ixodes persulcatus. A total of 28.8% of the ticks tested positive for either Rickettsia raoultii or "Candidatus Rickettsia tarasevichiae", in which 37.6% of the D. nuttalli, D. silvarum and H. longicornis ticks were positive for R. raoultii while 22.8% of the I. persulcatus ticks were positive for "Ca. R. tarasevichiae". The positive rate of both R. raoultii and "Ca. R. tarasevichiae" in ticks from the livestock (40.7%) was significantly higher than that from the vegetation (19.5%). All the data indicate that ticks in the Harbin area have a high infection rate with Rickettsia species and domestic animals may have a tick-livestock rickettsial circulation that may play an important role in transmission of rickettsioses.