Biodegradable calcium carbonate/mesoporous silica/poly(lactic-glycolic acid) microspheres scaffolds with osteogenesis ability for bone regeneration
Open Access
- 29 January 2021
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in RSC Advances
- Vol. 11 (9), 5055-5064
- https://doi.org/10.1039/d0ra09958a
Abstract
Sintered microsphere-based scaffolds provide a porous structure and high-resolution spatial organization control, show great potential for bone regeneration, mainly from biodegradable biomaterials including poly(lactic-glycolic acid) (PLGA). However, acidic monomer regeneration, mainly from biodegradable biomaterials including poly(lactic-glycolic acid) (PLGA). However, acidic monomers generated by PLGA degradation tend to cause tissue inflammation, which is the central issue of PLGA-based bone regeneration scaffolds development. In this work, calcium carbonate (CC)/hexagonal mesoporous silica (HMS)/PLGA sintered microsphere-based scaffolds were developed. The scaffolds possessed a three-dimensional (3D) network structure and 30–40% porosity. The degradation results indicated that CC/HMS/PLGA scaffolds could compensate for pH increased caused by PLGA acidic byproducts effectively. Degradation results showed that CC/HMS/PLGA scaffold could effectively compensate for the pH increase caused by PLGA acidic by-products. Composite CC additives can induce the increase of adhesive proteins in the environment, which is conducive to the adhesion of cells to scaffolds. Mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation were evaluated by CCK-8 assay, alkaline phosphatase (ALP) activity, ALP staining, and Alizarin Red staining. The results showed that compared with HMS/PLGA scaffolds, the proliferation of MSCs cultured with CC/HMS/PLGA scaffolds was enhanced. When cultured on the CC/HMS/PLGA scaffolds, MSCs also showed significantly enhanced ALP activity and higher calcium secretion compared with the HMS/PLGA scaffolds. CC/HMS/PLGA sintered microsphere-based scaffolds provides an attractive strategy for bone repair and regeneration with better performance.Funding Information
- National Natural Science Foundation of China (32000964)
- Guangdong Academy of Sciences (2020GDASYL-20200103038, 2021GDASYL-20210103026, 2019GDASYL-20210102, 2017GDASCX-0103)
- Guangdong Science and Technology Department (2020B1111560001, 2017A070701019)
- Guangzhou Science, Technology and Innovation Commission (201904010280)
This publication has 38 references indexed in Scilit:
- Application of open porous poly(D,L‐lactide‐co‐glycolide) microspheres and the strategy of hydrophobic seeding in hepatic tissue cultivationJournal of Biomedical Materials Research Part A, 2013
- The Use of Micro- and Nanospheres as Functional Components for Bone Tissue RegenerationTissue Engineering, Part B: Reviews, 2012
- Poly(lactide‐co‐glycolide)/titania composite microsphere‐sintered scaffolds for bone tissue engineering applicationsJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2010
- Functionalization of chitosan/poly(lactic acid‐glycolic acid) sintered microsphere scaffolds via surface heparinization for bone tissue engineeringJournal of Biomedical Materials Research Part A, 2009
- Modular tissue engineering: engineering biological tissues from the bottom upSoft Matter, 2009
- Formation of porous PLGA scaffolds by a combining method of thermally induced phase separation and porogen leachingJournal of Applied Polymer Science, 2008
- Poly(lactide-co-glycolide) microspheres as a moldable scaffold for cartilage tissue engineeringBiomaterials, 2005
- Tissue Engineering--Current Challenges and Expanding OpportunitiesScience, 2002
- Bone marrow induced osteogenesis in hydroxyapatite and calcium carbonate implantsBiomaterials, 1996
- Bone formation processin porous calcium carbonate and hydroxyapatiteJournal of Biomedical Materials Research, 1992