A plant‐specific SWR1 chromatin‐remodeling complex couples histone H2A.Z deposition with nucleosome sliding
- 2 March 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 39 (7), e102008
- https://doi.org/10.15252/embj.2019102008
Abstract
Deposition of H2A.Z in chromatin is known to be mediated by a conserved SWR1 chromatin‐remodeling complex in eukaryotes. However, little is known about whether and how the SWR1 complex cooperates with other chromatin regulators. Using immunoprecipitation followed by mass spectrometry, we found all known components of the Arabidopsis thaliana SWR1 complex and additionally identified the following three classes of previously uncharacterized plant‐specific SWR1 components: MBD9, a methyl‐CpG‐binding domain‐containing protein; CHR11 and CHR17 (CHR11/17), ISWI chromatin remodelers responsible for nucleosome sliding; and TRA1a and TRA1b, accessory subunits of the conserved NuA4 histone acetyltransferase complex. MBD9 directly interacts with CHR11/17 and the SWR1 catalytic subunit PIE1, and is responsible for the association of CHR11/17 with the SWR1 complex. MBD9, TRA1a, and TRA1b function as canonical components of the SWR1 complex to mediate H2A.Z deposition. CHR11/17 are not only responsible for nucleosome sliding but also involved in H2A.Z deposition. These results indicate that the association of the SWR1 complex with CHR11/17 may facilitate the coupling of H2A.Z deposition with nucleosome sliding, thereby co‐regulating gene expression, development, and flowering time.Keywords
This publication has 66 references indexed in Scilit:
- Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1Cell, 2013
- New insights into nucleosome and chromatin structure: an ordered state or a disordered affair?Nature Reviews Molecular Cell Biology, 2012
- Characterization of MADS-domain transcription factor complexes in Arabidopsis flower developmentProceedings of the National Academy of Sciences of the United States of America, 2012
- Recognition of a Mononucleosomal Histone Modification Pattern by BPTF via Multivalent InteractionsCell, 2011
- Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell IdentitiesMolecular Cell, 2010
- N Terminus of Swr1 Binds to Histone H2AZ and Provides a Platform for Subunit Assembly in the Chromatin Remodeling ComplexOnline Journal of Public Health Informatics, 2009
- Histone H2A.Z and DNA methylation are mutually antagonistic chromatin marksNature, 2008
- Translational and rotational settings of H2A.Z nucleosomes across the Saccharomyces cerevisiae genomeNature, 2007
- Repression of Flowering inArabidopsisRequires Activation ofFLOWERING LOCUS CExpression by the Histone Variant H2A.ZTHE PLANT CELL ONLINE, 2007
- SEF, a New Protein Required for Flowering Repression in Arabidopsis, Interacts with PIE1 and ARP6Plant Physiology, 2006