Analytical strategy for studying the formation and stability of multilayered films containing gold nanoparticles
- 26 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 413 (5), 1473-1483
- https://doi.org/10.1007/s00216-020-03113-6
Abstract
The design of layer-by-layer (LbL) polyelectrolyte films including nanoparticles is a growing field of innovation in a wide range of biomedical applications. Gold nanoparticles (AuNPs) are very attractive for further biomolecule coupling to induce a pharmacological effect. Nanostructured LbL films coupled with such metallic species show properties that depend on the conditions of construction, i.e. the polymer nature and dissolution buffer. Tripartite LbL films (polycation, AuNP, and polyanion) were evaluated using two different polycationic polymers (poly(allylamine hydrochloride) (PAH), poly(ethylene imine) (PEI)) and various medium conditions (salts, i.e. phosphate, Tris or Tris-NaCl buffers, and concentration). AuNP incorporation and film stability were analysed by visible spectrophotometry, capillary zone electrophoresis, a quartz crystal microbalance, and high-performance liquid chromatography. The ideal compromise between AuNP loading and film stability was obtained using PAH prepared in Tris-NaCl buffer (0.01–0.15 M). This condition allowed the formation of a LbL film that was more stable than the film with PEI and provided an AuNP quantity that was 4.8 times greater than that of the PAH-PBS-built film. In conclusion, this work presents an analytical strategy for the characterization of nanostructured multilayer films and optimization of LbL films enriched with AuNPs to design biomedical device coatings.Keywords
This publication has 25 references indexed in Scilit:
- Nanotechnologies for Medical Devices: Potentialities and RisksACS Applied Bio Materials, 2018
- Study of the Impact of Polyanions on the Formation of Lipid Bilayers on Top of Polyelectrolyte Multilayers with Poly(allylamine hydrochloride) as the Top LayerThe Journal of Physical Chemistry B, 2017
- The effect of top-layer chemistry on the formation of supported lipid bilayers on polyelectrolyte multilayers: primary versus quaternary aminesPhysical Chemistry Chemical Physics, 2016
- Electrodeposition of chitosan/gelatin/nanosilver: A new method for constructing biopolymer/nanoparticle composite films with conductivity and antibacterial activityMaterials Science and Engineering: C, 2015
- Antibacterial antiadhesion membranes from silver‐nanoparticle‐doped electrospun poly(L‐lactide) nanofibersJournal of Applied Polymer Science, 2013
- Optimization of Cardiovascular Stent against Restenosis: Factorial Design-Based Statistical Analysis of Polymer Coating ConditionsPLOS ONE, 2012
- Polymer assemblies for controlled delivery of bioactive molecules from surfacesAdvanced Drug Delivery Reviews, 2011
- Antibacterial activity of pH-sensitive genipin cross-linked chitosan/poly(ethylene glycol)/silver nanocompositesPolymers for Advanced Technologies, 2010
- MAD (Multiagent Delivery) Nanolayer: Delivering Multiple Therapeutics from Hierarchically Assembled Surface CoatingsLangmuir, 2009
- Multicompartment Films Made of Alternate Polyelectrolyte Multilayers of Exponential and Linear GrowthLangmuir, 2004