Evaluation of the procoagulant properties of a newly developed platelet modified lysate product
- 15 May 2020
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 60 (7), 1579-1589
- https://doi.org/10.1111/trf.15844
Abstract
BACKGROUND Platelet transfusion is associated with logistical problems with the national storage guidelines of platelets. This results in decreased function in vivo as a result of the platelet storage lesion, and complications such as allergic or hemolytic reactions and thrombosis. We evaluated a new, freshly prepared platelet modified lysate (PML) product designed to be more procoagulant than fresh and stored platelets. METHODS Fresh platelets were concentrated, sonicated, and centrifuged to produce PML. Samples of both washed and unwashed PML were evaluated for particle size, concentration, and activity, and then tested for clot kinetics and thrombin generation. PML samples were also stored at various temperatures for durations up to 6 months and evaluated for clot kinetics and thrombin generation throughout. RESULTS PML showed significantly higher concentration of platelet microparticles, increased procoagulant properties, and increased thrombin generation as compared to fresh and stored platelets. In addition, PML maintained its clot kinetics over a 6‐month storage period with variable storage conditions. CONCLUSIONS The newly proposed PML product is more procoagulant, stable, and has additional potential applications than currently available platelet products. Further studies will be performed to assess its functions in vivo and to assess thrombotic potential.This publication has 37 references indexed in Scilit:
- Topical hemostatic agents in surgical practiceTransfusion and Apheresis Science, 2011
- Platelet Lysate Mucohadesive Formulation to Treat Oral Mucositis in Graft Versus Host Disease Patients: A New Therapeutic ApproachAAPS PharmSciTech, 2011
- Deciphering the human platelet sheddomeBlood, 2011
- Transfusion protocol in traumaJournal of Emergencies, Trauma, and Shock, 2011
- Clinical relevance of microparticles from platelets and megakaryocytesCurrent Opinion in Hematology, 2010
- MicroparticlesCirculation Research, 2010
- Platelet transfusions: trigger, dose, benefits, and risksF1000 Medicine Reports, 2010
- Blood Transfusions, Thrombosis, and Mortality in Hospitalized Patients With CancerArchives of Internal Medicine, 2008
- Platelet lysate promotes in vitro wound scratch closure of human dermal fibroblasts: different roles of cell calcium, P38, ERK and PI3K/AKTJournal of Cellular and Molecular Medicine, 2008