Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus
Open Access
- 23 June 2020
- Vol. 9 (6), 509
- https://doi.org/10.3390/pathogens9060509
Abstract
Soil-borne pathogens can have considerable detrimental effects on asparagus (Asparagus officinalis) growth and production, notably caused by the Fusarium species F. oxysporum f.sp. asparagi, F. proliferatum and F. redolens. In this study, their species-specific impact regarding disease severity and root morphological traits was analysed. Additionally, various isolates were characterised based on in vitro physiological activities and on protein extracts using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The response of two asparagus cultivars to the different Fusarium species was evaluated by inoculating experiments. Differences in aggressiveness were observed between Fusarium species and their isolates on roots, while no clear disease symptoms became visible in ferns eight weeks after inoculation. F. redolens isolates Fred1 and Fred2 were the most aggressive strains followed by the moderate aggressive F. proliferatum and the less and almost non-aggressive F. oxysporum isolates, based on the severity of disease symptoms. Fungal DNA in stem bases and a significant induction of pathogenesis-related gene expression was detectable in both asparagus cultivars. A significant negative impact of the pathogens on the root characteristics total root length, volume, and surface area was detected for each isolate tested, with Fred1 causing the strongest effects. No significant differences between the tested asparagus cultivars were observed.Keywords
This publication has 59 references indexed in Scilit:
- Comparative analysis of fungal genomes reveals different plant cell wall degrading capacity in fungiBMC Genomics, 2013
- Advancements in the Analysis of the Arabidopsis Plasma Membrane ProteomeFrontiers in Plant Science, 2013
- The xylem as battleground for plant hosts and vascular wilt pathogensFrontiers in Plant Science, 2013
- Activities of cell wall degrading enzymes in autolyzing cultures of threeFusarium culmorumisolates: growth-promoting, deleterious and pathogenic to rye (Secale cereale)Mycologia, 2011
- Comparisons of treatment means when factors do not interact in two-factorial studiesAmino Acids, 2011
- PCR-SSCP analysis of Fusarium diversity in asparagus decline in JapanEuropean Journal of Plant Pathology, 2011
- Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR dataNucleic Acids Research, 2009
- Mycotoxins Biosynthesis by Fusarium Oxysporum and F. Proliferatum Isolates of Asparagus OriginJournal of Plant Protection Research, 2009
- A PCR-denaturing gradient gel electrophoresis approach to assess Fusarium diversity in asparagusJournal of Microbiological Methods, 2005
- Biological Control of Fusarium oxysporum f.sp. asparagi by Applying Non-pathogenic Isolates of F . oxysporumBiocontrol Science and Technology, 1997