Campylobacter coli strains from Brazil can invade phagocytic and epithelial cells and induce IL-8 secretion
- 15 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Brazilian Journal of Microbiology
- Vol. 52 (2), 859-867
- https://doi.org/10.1007/s42770-021-00450-1
Abstract
Campylobacter spp. have been a predominant cause of bacterial foodborne gastroenteritis worldwide, causing substantial costs to public healthcare systems. This study aimed to assess the invasion and pro-inflammatory cytokine production capacity of Campylobacter coli strains isolated in Brazil. A total of 50 C. coli isolated from different sources in Brazil were analyzed for their capacity of invasion in Caco-2 and U-937 cell lines. The production of pro-inflammatory cytokines was quantitatively measured in response to C. coli. All the strains studied showed invasion percentage ≥ 40% in polarized Caco-2 cells. In U-937 cells assay, 35 of 50 C. coli strains studied showed invasion percentage ≥ 50%. A significant increase in IL-8 production by infected U-937 cells was observed for 17.5% of the C. coli isolates. The high percentages of invasion in Caco-2 and U-937 cells observed for all studied strains, plus the increased production of IL-8 by U-937 cells against some strains, highlighted the pathogenic potential of the C. coli studied and bring extremely relevant data since it has never been reported for strains isolated in Brazil and there are a few data for C. coli in the literature.Keywords
Funding Information
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2016/24716-3, 2015/23408-0)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (001)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (304399/2018-3)
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