Increased Level of Long Non-Coding RNA MALAT1 Is a Common Feature of Amoeboid Invasion
Open Access
- 1 May 2020
- Vol. 12 (5), 1136
- https://doi.org/10.3390/cancers12051136
Abstract
The ability of cancer cells to adopt various migration modes (the plasticity of cancer cell invasiveness) is a substantive obstacle in the treatment of metastasis, yet still an incompletely understood process. We performed a comparison of publicly available transcriptomic datasets from various cell types undergoing a switch between the mesenchymal and amoeboid migration modes. Strikingly, lncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) was one of three genes that were found upregulated in all amoeboid cells analyzed. Accordingly, downregulation of MALAT1 in predominantly amoeboid cell lines A375m2 and A2058 resulted in decrease of active RhoA (Ras homolog family member A) and was accompanied by the amoeboid-mesenchymal transition in A375m2 cells. Moreover, MALAT1 downregulation in amoeboid cells led to increased cell proliferation. Our work is the first to address the role of MALAT1 in MAT/AMT (mesenchymal to amoeboid transition/amoeboid to mesenchymal transition) and suggests that increased MALAT1 expression is a common feature of amoeboid cells.This publication has 55 references indexed in Scilit:
- Metastasis of aggressive amoeboid sarcoma cells is dependent on Rho/ROCK/MLC signalingCell Communication and Signaling, 2013
- Loss of the abundant nuclear non-coding RNAMALAT1is compatible with life and developmentRNA Biology, 2012
- The lncRNA Malat1 Is Dispensable for Mouse Development but Its Transcription Plays a cis-Regulatory Role in the AdultCell Reports, 2012
- Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear specklesRNA, 2012
- Noncoding RNA gene silencing through genomic integration of RNA destabilizing elements using zinc finger nucleasesGenome Research, 2011
- The role of the tissue microenvironment in the regulation of cancer cell motility and invasionCell Communication and Signaling, 2010
- The molecular mechanisms of transition between mesenchymal and amoeboid invasiveness in tumor cellsCellular and Molecular Life Sciences, 2009
- The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR ExperimentsClinical Chemistry, 2009
- 3′ End Processing of a Long Nuclear-Retained Noncoding RNA Yields a tRNA-like Cytoplasmic RNACell, 2008
- Rac Activation and Inactivation Control Plasticity of Tumor Cell MovementCell, 2008