Acoustofluidic rotational tweezing enables high-speed contactless morphological phenotyping of zebrafish larvae
Open Access
- 18 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 12 (1), 1-13
- https://doi.org/10.1038/s41467-021-21373-3
Abstract
Modern biomedical research and preclinical pharmaceutical development rely heavily on the phenotyping of small vertebrate models for various diseases prior to human testing. In this article, we demonstrate an acoustofluidic rotational tweezing platform that enables contactless, high-speed, 3D multispectral imaging and digital reconstruction of zebrafish larvae for quantitative phenotypic analysis. The acoustic-induced polarized vortex streaming achieves contactless and rapid (~1 s/rotation) rotation of zebrafish larvae. This enables multispectral imaging of the zebrafish body and internal organs from different viewing perspectives. Moreover, we develop a 3D reconstruction pipeline that yields accurate 3D models based on the multi-view images for quantitative evaluation of basic morphological characteristics and advanced combinations of metrics. With its contactless nature and advantages in speed and automation, our acoustofluidic rotational tweezing system has the potential to be a valuable asset in numerous fields, especially for developmental biology, small molecule screening in biochemistry, and pre-clinical drug development in pharmacology.Keywords
Funding Information
- Foundation for the National Institutes of Health (R01GM132603, R01GM135486, R33CA223908, R01GM127714, R43OD024963)
- National Science Foundation (ECCS-1807601)
This publication has 71 references indexed in Scilit:
- High-throughput hyperdimensional vertebrate phenotypingNature Communications, 2013
- Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologiesProceedings of the National Academy of Sciences of the United States of America, 2012
- Drinks Like a Fish: Using Zebrafish to Understand Alcoholic Liver DiseaseAlcohol: Clinical and Experimental Research, 2011
- High-throughput in vivo vertebrate screeningNature Methods, 2010
- Monitoring neural activity with bioluminescence during natural behaviorNature Neuroscience, 2010
- Micro-computed tomography-based phenotypic approaches in embryology: procedural artifacts on assessments of embryonic craniofacial growth and developmentBMC Developmental Biology, 2010
- Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regenerationDevelopmental Biology, 2009
- Hepatic steatosis in response to acute alcohol exposure in zebrafish requires sterol regulatory element binding protein activationJournal of Hepatology, 2008
- Generating Transparent Zebrafish: A Refined Method to Improve Detection of Gene Expression During Embryonic DevelopmentMarine Biotechnology, 2001
- Acoustic streamingJournal of Sound and Vibration, 1978