Assessment of Reproductive Toxicity of Hydroethanolic Root Extracts of Caesalpinia benthamiana, Sphenocentrum jollyanum, and Paullinia pinnata
Open Access
- 1 March 2021
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Experimental Pharmacology
- Vol. ume 13, 223-234
- https://doi.org/10.2147/jep.s283557
Abstract
Purpose: Male sexual dysfunction negatively affects an individual’s quality of life and thus its of prime public concern, hence the need to boost reproductive abilities in such individuals. This study assessed the effect of hydroethanolic root extracts of Caesalpinia benthamiana (CBRE), Sphenocentrum jollyanum (SJRE), and Paullinia pinnata (PPRE), commonly used as aphrodisiacs in Ghana, using male Sprague-Dawley rats. Methods: Plasma testosterone, follicle-stimulating hormone, and luteinizing hormone were assayed in grouped rats treated orally with 1 mL/kg normal saline, 50 mg/kg monosodium glutamate (MSG), and 100, 300, or 1000 mg/kg CBRE, SJRE, and PPRE, respectively, for 60 days. Epididymis and testis weights were determined. Semen was assessed on spermatozoa count, motility, and morphology. Malonyladehyde formation in lipid-peroxidation assay and histological examinations were performed to assess pathological changes in testes. Testicular testosterone was also assayed. Results: While MSG, CBRE, SJRE, and PPRE treatments did not result in significant reduction (p> 0.05) in plasma testosterone, there was significant reduction (p≤ 0.05 − 0.0001) in plasma luteinizing hormone, and follicle-stimulating hormone. The combined mean wet weights of epididymides and testes of all treated groups did not vary significantly (p> 0.05) from the control. There was significant reduction (p≤ 0.0001) in sperm motility and count, with significant morphological changes (p≤ 0.05– 0.001), ie, bent necks, tails, and midpieces, and multiple anomalies in the spermatozoa in extract and MSG-treated groups. There was also significant (p≤ 0.0001) reduction in testicular testosterone among all treatment groups. Conclusion: Hydroethanolic CBRE, SJRE, and PPRE were found to have detrimental effects on reproductive function with prolonged usage and thus may not be safe to use in healthy males who intend to reproduce.Keywords
This publication has 32 references indexed in Scilit:
- Hormonal regulation of male germ cell developmentJournal of Endocrinology, 2010
- Incidence of sexual dysfunction: a prospective survey in Ghanaian femalesReproductive Biology and Endocrinology, 2010
- Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantificationQuímica Nova, 2009
- Antioxidant Properties of the Methanol Extracts from the Leaves of Paullinia pinnataJournal of Medicinal Food, 2007
- Relationships Between Serum Hormone Levels and Semen Quality Among Men From an Infertility ClinicJournal of Andrology, 2007
- FSH regulates the formation of adherens junctions and ectoplasmic specialisations between rat Sertoli cells in vitro and in vivoJournal of Endocrinology, 2006
- Paullinia pinnata Extracts Rich in Polyphenols Promote Vascular Relaxation via Endothelium-dependent MechanismsJournal of Cardiovascular Pharmacology, 2006
- Comparative Toxicity of Five Commonly Used Analgesics on Rat Sperm Count and Sperm MorphologyGlobal Journal of Pure and Applied Sciences, 2005
- ‘Viagra® Effect’ – Influence of Mass Media on Patient BehaviorUrologia Internationalis, 2001
- Critical Role of Reactive Oxygen Species and Mitochondrial Permeability Transition in Microcystin-Induced Rapid Apoptosis in Rat HepatocytesHepatology, 2000