Molecular and Cellular Responses of DNA Methylation and Thioredoxin System to Heat Stress in Meat-Type Chickens
Open Access
- 30 June 2021
- Vol. 11 (7), 1957
- https://doi.org/10.3390/ani11071957
Abstract
Heat stress (HS) causes molecular dysfunction that adversely affects chicken performance and increases mortality. The responses of chickens to HS are extremely complex. Thus, the aim of this study was to evaluate the influence of acute and chronic exposure to HS on the expression of thioredoxin–peroxiredoxin system genes and DNA methylation in chickens. Chickens at 14 d of age were divided into two groups and reared under either constant normal temperature (25 °C) or high temperature (35 °C) in individual cages for 12 days. Five birds per group at one and 12 days post-HS were euthanized and livers were sampled for gene expression. The liver and Pectoralis major muscle were sampled for cellular analysis. mRNA expression of thioredoxin and peroxiredoxins (Prdx) 1, 3, and 4 in the liver were down-regulated at 12 days post-HS compared to controls. The liver activity of thioredoxin reductase (TXNRD) and levels of peroxiredoxin1 (Prdx1) at 12 days post-HS were significantly decreased. The results reveal that there was a significant decrease in DNA methylation at 12 days post HS in liver tissues. In conclusion, pathway of thioredoxin system under HS may provide clues to nutritional strategies to mitigate the effect of HS in meat-type chicken.Keywords
Funding Information
- Advanced Technologies for the Genetic Improvement of Poultry from the USDA National Institute of Food and Agriculture (project accession no. 1025875)
This publication has 33 references indexed in Scilit:
- Crucial Role of Membrane Potential in Heat Stress-Induced Overproduction of Reactive Oxygen Species in Avian Skeletal Muscle MitochondriaPLOS ONE, 2013
- Mitochondrial Thiols in Antioxidant Protection and Redox Signaling: Distinct Roles for Glutathionylation and Other Thiol ModificationsAntioxidants and Redox Signaling, 2012
- Comparison of Gene Expression and Genome-Wide DNA Methylation Profiling between Phenotypically Normal Cloned Pigs and Conventionally Bred ControlsPLOS ONE, 2011
- Changes in the expression of mitochondrial peroxiredoxin and thioredoxin in neurons and glia and their protective effects in experimental cerebral ischemic damageFree Radical Biology & Medicine, 2010
- Epigenetics: A Landscape Takes ShapeCell, 2007
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Peroxiredoxin, a Novel Family of PeroxidasesIUBMB Life, 2001
- Thioredoxin reductaseJBIC Journal of Biological Inorganic Chemistry, 2000
- Mammalian Peroxiredoxin Isoforms Can Reduce Hydrogen Peroxide Generated in Response to Growth Factors and Tumor Necrosis Factor-αJournal of Biological Chemistry, 1998
- Thioredoxin regenerates proteins inactivated by oxidative stress in endothelial cellsJBIC Journal of Biological Inorganic Chemistry, 1992