Babao Dan Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway
- 9 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Chinese Journal of Integrated Medicine
- Vol. 28 (11), 1000-1006
- https://doi.org/10.1007/s11655-021-3282-0
Abstract
Abstract Objective To evaluate the protective function of Babao Dan (八宝丹, BBD) on 5-flurouracil (5-FU)-induced intestinal mucositis (IM) and uncover the underlying mechanism. Methods A total of 18 male mice were randomly divided into 3 groups by a random number table, including control, 5-FU and 5-FU combined BBD groups, 6 mice in each group. A single intraperitoneal injection of 5-FU (150 mg/kg) was performed in 5-FU and 5-FU combined BBD groups on day 0. Mice in 5-FU combined BBD group were gavaged with BBD (250 mg/kg) daily from day 1 to 6. Mice in the control group were gavaged with saline solution for 6 days. The body weight and diarrhea index of mice were recorded daily. On the 7th day, the blood from the heart of mice was collected to analyze the proportional changes of immunological cells, and the mice were subsequently euthanized by mild anesthesia with 2% pentobarbital sodium. Colorectal lengths and villus heights were measured. Intestinal-cellular apoptosis and proliferation were evaluated by Tunel assay and immunohistochemical staining of proliferating cell nuclear antigen, respectively. Immunohistochemistry and Western blot were performed to investigate the expressions of components in Wnt/β-catenin pathway (Wnt3, LRP5, β-catenin, c-Myc, LRG5 and CD44). Results BBD obviously alleviated 5-FU-induced body weight loss and diarrhea, and reversed the decrease in the number of white blood cells, including monocyte, granulocyte and lymphocyte, and platelet (PPP Conclusions BBD attenuates the adverse effects induced by 5-FU via Wnt/β-catenin pathway, suggesting it may act as a potential agent against chemotherapy-induced intestinal mucositis.Keywords
This publication has 28 references indexed in Scilit:
- Rebamipide Attenuates 5-Fluorouracil-Induced Small Intestinal Mucositis in a Mouse ModelBiological & Pharmaceutical Bulletin, 2015
- MYC and the Control of ApoptosisCold Spring Harbor Perspectives in Medicine, 2014
- Stem cell dynamics in homeostasis and cancer of the intestineNature Reviews Cancer, 2014
- Inhibitory Effect of the Gallotannin Corilagin on Dextran Sulfate Sodium-Induced Murine Ulcerative ColitisJournal of Natural Products, 2013
- Redundant Sources of Wnt Regulate Intestinal Stem Cells and Promote Formation of Paneth CellsGastroenterology, 2012
- A Novel Tankyrase Inhibitor Decreases Canonical Wnt Signaling in Colon Carcinoma Cells and Reduces Tumor Growth in Conditional APC Mutant MiceCancer Research, 2012
- 5-Fluorouracil Induced Intestinal Mucositis via Nuclear Factor-κB Activation by Transcriptomic Analysis and In Vivo Bioluminescence ImagingPLOS ONE, 2012
- Intestinal development and differentiationExperimental Cell Research, 2011
- Wnt Signaling, Lgr5, and Stem Cells in the Intestine and SkinThe American Journal of Pathology, 2009
- Haematological and hepatotoxic effects of silken styles of corn in albino ratsJournal of Applied Toxicology, 1992