Linc‐ROR promotes the progression of breast cancer and decreases the sensitivity to rapamycin through miR‐194‐3p targeting MECP2
Open Access
- 26 April 2020
- journal article
- research article
- Published by Wiley in Molecular Oncology
- Vol. 14 (9), 2231-2250
- https://doi.org/10.1002/1878-0261.12700
Abstract
Linc‐ROR is reported to be a potential biomarker of breast cancer, but the detailed mechanism of linc‐ROR‐mediated breast cancer regulation has not been fully studied. We aimed to explore how linc‐ROR affects proliferation, metastasis and drug sensitivity in breast cancer. Cell lines in which linc‐ROR was overexpressed or knocked down were constructed, and the cell proliferation, colony formation, cell migration and invasion abilities of these lines were explored. A CCK‐8 assay was performed to determine the sensitivity of the breast cancer cells to rapamycin. Next‐generation sequencing was conducted to explore the detailed regulatory mechanism of linc‐ROR; differentially expressed RNAs in the linc‐ROR‐overexpressing cell line compared with the negative control were screened out and their target genes were chosen to perform Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, protein‐protein interaction (PPI) network analysis and competing endogenous RNA (ceRNA) network analysis. The ceRNA mechanism of linc‐ROR for miR‐194‐3p, which targets MECP2, was determined through dual‐luciferase reporter assay, RT‐qPCR, western blot and rescue experiments. Finally, we found that linc‐ROR was upregulated in breast tumor tissues. Linc‐ROR promoted the cell proliferation, colony formation, cell migration and invasion of breast cancer and decreased the sensitivity of breast cancer cells to rapamycin. The overexpression of linc‐ROR triggered changes in the whole transcriptome of breast cancer cells, and a total of 85 lncRNAs, 414 microRNAs, 490 mRNAs and 92 circRNAs were differentially expressed in the linc‐ROR‐overexpressing cell line compared with the negative control. Through a series of bioinformatics analyses, the “linc‐ROR/miR‐194‐3p/MECP2” ceRNA regulatory axis was confirmed to be involved in the linc‐ROR‐mediated progression and drug sensitivity of breast cancer. In conclusion, linc‐ROR serves as an onco‐lncRNA in breast cancer and promotes the survival of breast cancer cells during rapamycin treatment by functioning as a ceRNA sponge for miR‐194‐3p, which targets MECP2.Keywords
This publication has 64 references indexed in Scilit:
- TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusionsGenome Biology, 2013
- Fast gapped-read alignment with Bowtie 2Nature Methods, 2012
- A two-stage association study identifies methyl-CpG-binding domain protein 2 gene polymorphisms as candidates for breast cancer susceptibilityEuropean Journal of Human Genetics, 2012
- p53-Responsive miR-194 Inhibits Thrombospondin-1 and Promotes Angiogenesis in Colon CancersCancer Research, 2011
- TopHat-Fusion: an algorithm for discovery of novel fusion transcriptsGenome Biology, 2011
- edgeR: a Bioconductor package for differential expression analysis of digital gene expression dataBioinformatics, 2009
- Origins and Mechanisms of miRNAs and siRNAsCell, 2009
- Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammalsNature, 2009
- Systematic and integrative analysis of large gene lists using DAVID bioinformatics resourcesNature Protocols, 2008
- Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction NetworksGenome Research, 2003