Horsing Around: Escherichia coli ST1250 of Equine Origin Harboring Epidemic IncHI1/ST9 Plasmid with bla CTX-M-1 and an Operon for Short-Chain Fructooligosaccharide Metabolism
- 19 April 2021
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 65 (5)
- https://doi.org/10.1128/aac.02556-20
Abstract
The relatedness of the equine-associated Escherichia coli ST1250 and its single- and double-locus variants (ST1250-SLV/DLV), obtained from horses in Europe, was studied by comparative genome analysis. A total of 54 isolates of E. coli ST1250 and ST1250-SLV/DLV from healthy and hospitalized horses across Europe [Czech Republic (n=23), the Netherlands (n=18), Germany (n=9), Denmark (n=3) and France (n=1)] from 2008-2017 were subjected to whole-genome sequencing. An additional 25 draft genome assemblies of E. coli ST1250 and ST1250-SLV/DLV were obtained from the public databases. The isolates were compared for genomic features, virulence genes, clade structure and plasmid content. The complete nucleotide sequences of eight IncHI1/ST9 and one IncHI1/ST2 plasmids were obtained using long-read sequencing by PacBio or MinION. In the collection of 79 isolates, only 10 were phylogenetically close (98% similarity) regardless of country of origin and varied only in the structure and integration site of MDR region. E. coli ST1250 and ST1250-SLV/DLV are phylogenetically-diverse strains associated with horses. A strong linkage of E. coli ST1250 with epidemic multi-drug resistance plasmid lineage IncHI1/ST9 carrying blaCTX-M-1 and the fos operon was identified.Funding Information
- Czech Science Foundation (18-23532S)
- Internal Grant Agency of University of Veterinary and Pharmaceutical Sciences Brno (204/2020/FVHE, 116/2015/FVL)
- Charles University Research Fund PROGRES (Q39)
- National Sustainability Program I (LO1503)
- Ministry of Education Youth and Sports of the Czech Republic (CZ.02.1.01/0.0/0.0/16_019/0000787)
- Horizon 2020 (773830)
- OH-EJP-H2020-JRP-AMR-2-ARDIG
- Utrecht University
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