Development of Biomimetic Alginate/Gelatin/Elastin Sponges with Recognition Properties toward Bioactive Peptides for Cardiac Tissue Engineering
Open Access
- 11 December 2020
- journal article
- research article
- Published by MDPI AG in Biomimetics
- Vol. 5 (4), 67
- https://doi.org/10.3390/biomimetics5040067
Abstract
In recent years, there has been an increasing interest toward the covalent binding of bioactive peptides from extracellular matrix proteins on scaffolds as a promising functionalization strategy in the development of biomimetic matrices for tissue engineering. A totally new approach for scaffold functionalization with peptides is based on Molecular Imprinting technology. In this work, imprinted particles with recognition properties toward laminin and fibronectin bioactive moieties were synthetized and used for the functionalization of biomimetic sponges, which were based on a blend of alginate, gelatin, and elastin. Functionalized sponges underwent a complete morphological, physicochemical, mechanical, functional, and biological characterization. Micrographs of functionalized sponges showed a highly porous structure and a quite homogeneous distribution of imprinted particles on their surface. Infrared and thermal analyses pointed out the presence of interactions between blend components. Biodegradation and mechanical properties appeared adequate for the aimed application. The results of recognition tests showed that the deposition on sponges did not alter the specific recognition and binding behavior of imprinted particles. In vitro biological characterization with cardiac progenitor cells showed that early cell adherence was promoted. In vivo analysis showed that developed scaffolds improved cardiac progenitor cell adhesion and differentiation toward myocardial phenotypes.Keywords
This publication has 37 references indexed in Scilit:
- To Remove or Not to Remove? The Challenge of Extracting the Template to Make the Cavities Available in Molecularly Imprinted Polymers (MIPs)International Journal of Molecular Sciences, 2011
- Growth Factor-Induced Mobilization of Cardiac Progenitor Cells Reduces the Risk of Arrhythmias, in a Rat Model of Chronic Myocardial InfarctionPLOS ONE, 2011
- High-resolution X-ray microtomography for three-dimensional imaging of cardiac progenitor cell homing in infarcted rat heartsJournal of Tissue Engineering and Regenerative Medicine, 2011
- Human cardiac and bone marrow stromal cells exhibit distinctive properties related to their originCardiovascular Research, 2010
- Protein binding to peptide-imprinted porous silica scaffoldsChemical Engineering Journal, 2008
- LAMININ FUNCTIONS IN TISSUE MORPHOGENESISAnnual Review of Cell and Developmental Biology, 2004
- Temperature, sample and time dependent structural characteristics of gelatine gels studied by near infrared spectroscopyFood Hydrocolloids, 2004
- Expression of α and β integrins during terminal differentiation of cardiomyocytesCardiovascular Research, 2000
- Cell Function on Substrates Containing Immobilized Bioactive PeptidesMRS Proceedings, 1993
- Role of Fibronectin in Adhesion, Migration, and MetastasisCancer Investigation, 1989