Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples
Open Access
- 8 June 2020
- Vol. 12 (6), 624
- https://doi.org/10.3390/v12060624
Abstract
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 min contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.Keywords
Funding Information
- H2020 Research Infrastructures (871029)
- H2020 Fast Track to Innovation (101003544)
- Agence Nationale de la Recherche (PREPMedVet)
- H2020 European Institute of Innovation and Technology (823666)
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