The pore size of chitosan-Aloe vera scaffold and its effect on VEGF expressions and woven alveolar bone healing of tooth extraction of Cavia cobaya
Open Access
- 15 September 2020
- journal article
- Published by Universitas Airlangga in Dental Journal (Majalah Kedokteran Gigi)
- Vol. 53 (3), 115-121
- https://doi.org/10.20473/j.djmkg.v53.i3.p115-121
Abstract
Background: Pore size of scaffolds affects cellular activity, stimulates angiogenetic factors of vascular endothelial growth factor (VEGF), synthesises new blood vessels to regulate migration and proliferation, and accelerates alveolar bone healing of tooth extraction. Purpose: This study aims to analyse the pore size of chitosan-Aloe vera scaffold and its effects on VEGF expression and woven alveolar bone healing of tooth extraction of Cavia cobaya. Methods: 36 male Cavia cobaya, aged 3-3.5 months were divided into six groups: negative control groups (without scaffold), positive control groups (chitosan scaffold), and treatment groups (chitosan-Aloe vera scaffold) on 7- and 14-day observations. Histopathological examination was performed to account the woven alveolar bone areas, and immunohistochemical examination was conducted to examine VEGF expressions on endothelial cells. Data was analysed using a one-way analysis of variance (ANOVA) and least significant difference (LSD) test (pResults: Chitosan-Aloe vera scaffold was found to have open pore interconnectivity, the largest pore size was 138.9 μm, while the smallest was 110.5 μm and average pore size was 134.85 μm. The highest expression of VEGF was observed in the treatment group on days 7 (11.5 ±1.39) and 14 (15.28±1.78), while the largest woven alveolar bone was observed in the treatment group on days 7(17.83±1.47) and 14 (37.67±3.65). Statistically, there was a significant difference between control groups and the treatment groups (p=0.000; pConclusion: Chitosan-Aloe vera scaffold has pore characteristics increasing VEGF expressions and woven alveolar bone areas.Keywords
This publication has 29 references indexed in Scilit:
- Grafts for Ridge PreservationJournal of Functional Biomaterials, 2015
- Expression of bone morphogenetic protein-2 after using chitosan gel with different molecular weight on wound healing process of dental extractionDental Journal (Majalah Kedokteran Gigi), 2015
- Bone Replacement Materials and Techniques Used for Achieving Vertical Alveolar Bone AugmentationMaterials, 2015
- Role of primary stability for successful osseointegration of dental implants: Factors of influence and evaluationInterventional Medicine and Applied Science, 2013
- An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicineActa Biomaterialia, 2013
- New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineeringDental Materials Journal, 2013
- The increasing of fibroblast growth factor 2, osteocalcin, and osteoblast due to the induction of the combination of Aloe vera and 2% xenograft concelous bovineDental Journal (Majalah Kedokteran Gigi), 2012
- Aloe vera in the treatment for oral submucous fibrosis – a preliminary studyJournal of Oral Pathology & Medicine, 2012
- Biomimetic nanofibrous scaffolds for bone tissue engineeringBiomaterials, 2011
- Histologic Analysis of Healing After Tooth Extraction With Ridge Preservation Using Mineralized Human Bone AllograftThe Journal of Periodontology, 2010