Private hospitals are an important part of China’s medical and health service system. They play an indispensable role in alleviating the operation pressure of public hospitals and improving China’s medical and health market. As private hospitals undertake the important task of treating patients and saving people and ensuring people’s health and life safety, how to improve the clinical medical care level of private hospitals has become the focus of the hospital itself and the society. Clinical nurse is the core factor that determines the level of clinical care in the hospital, which affects the quality of clinical service and clinical treatment effect of the hospital. By establishing the training system of clinical nurses, scientifically promoting the training of clinical nurses, and enhancing the professional quality and professional ability of clinical nurses, it can effectively promote the improvement of the clinical medical level of private hospitals, and enable private hospitals to better implement their mission and responsibility of treating patients and saving people and serving the health of the masses. Based on the author’s own practical work experience and learning understanding, this paper first analyzes the necessity of establishing the training system of clinical nurses in private hospitals from different angles, and then puts forward some exploratory suggestions on the establishment of the training system of clinical nurses in private hospitals, mainly taking a private hospital as an example.