Bacillus subtilis SL18r Induces Tomato Resistance Against Botrytis cinerea, Involving Activation of Long Non-coding RNA, MSTRG18363, to Decoy miR1918
Open Access
- 3 February 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Plant Science
Abstract
Mounting evidence has indicated that beneficial rhizobacteria can suppress foliar pathogen invasion via elicitation of induced systemic resistance (ISR). However, it remains elusive whether long non-coding RNAs (lncRNAs) are involved in the mediation of the rhizobacteria-primed ISR processes in plants. Herein, we demonstrated the ability of the rhizobacterial strain Bacillus subtilis SL18r to trigger ISR in tomato plants against the foliar pathogen Botrytis cinerea. Comparative transcriptome analysis was conducted to screen differentially expressed lncRNAs (DELs) between the non-inoculated and SL18r-inoculated plants. Among these DELs, four variants of MSTRG18363 possessed conserved binding sites for miR1918, which negatively regulates immune systems in tomato plants. The expression of MSTRG18363 in tomato leaves was significantly induced by SL18r inoculation. The transcription of MSTRG18363 was negatively correlated with the expression of miR1918, but displayed a positive correlation with the transcription of the RING-H2 finger gene SlATL20 (a target gene of miR1918). Moreover, MSTRG18363-overexpressing plants exhibited the enhanced disease resistance, reduction of miR1918 transcripts, and marked increases of SlATL20 expression. However, the SL18r-induced disease resistance was largely impaired in the MSTRG18363-silenced plants. VIGS-mediated SlATL20 silencing also greatly weakened the SL18r-induced disease resistance. Collectively, our results suggested that induction of MSTRG18363 expression in tomato plants by SL18r was conducive to promoting the decoy of miR1918 and regulating the expression of SlATL20, thereby provoking the ISR responses against foliar pathogen infection.This publication has 56 references indexed in Scilit:
- RNA decoysPlant Signaling & Behavior, 2012
- VpRFP1, a novel C4C4-type RING finger protein gene from Chinese wild Vitis pseudoreticulata, functions as a transcriptional activator in defence response of grapevineJournal of Experimental Botany, 2011
- The Pepper E3 Ubiquitin Ligase RING1 Gene,CaRING1, Is Required for Cell Death and the Salicylic Acid-Dependent Defense ResponsePlant Physiology, 2011
- Botrytis cinerea Manipulates the Antagonistic Effects between Immune Pathways to Promote Disease Development in TomatoTHE PLANT CELL ONLINE, 2011
- Differential expression analysis for sequence count dataGenome Biology, 2010
- Abscisic Acid Deficiency Causes Changes in Cuticle Permeability and Pectin Composition That Influence Tomato Resistance to Botrytis cinereaPlant Physiology, 2010
- TopHat: discovering splice junctions with RNA-SeqBioinformatics, 2009
- Pseudomonas fluorescens WCS374r-Induced Systemic Resistance in Rice against Magnaporthe oryzae Is Based on Pseudobactin-Mediated Priming for a Salicylic Acid-Repressible Multifaceted Defense ResponsePlant Physiology, 2008
- Herbivore-Induced Resistance against Microbial Pathogens in ArabidopsisPlant Physiology, 2006
- Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol componentsPlant Molecular Biology Reporter, 1997