Treadmill exercise facilitated rotator cuff healing is coupled with regulating periphery neuropeptides expression in a murine model
- 1 April 2020
- journal article
- research article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 39 (3), 680-692
- https://doi.org/10.1002/jor.24678
Abstract
Postoperative exercise has been found able to accelerate bone‐tendon (B‐T) healing. In this study, we systematically compared tendon‐to‐bone healing in mice subjected to postoperative treadmill exercise and free cage recovery in a murine rotator cuff repair model. Specifically, C57BL/6 mice underwent unilateral supraspinatus tendon (SST) detachment and repair were randomly allocated into treadmill group and control group. Treadmill group received daily treadmill running initiated from postoperative day 7 while the control group was allowed free cage activity. Mice were euthanized at postoperative 4 and 8 weeks for synchrotron radiation micro‐computed tomography (SR‐μCT), histology and biomechanical tests to investigate the effect of treadmill running on B‐T healing. The results indicated that treadmill running initiated at day 7 postoperatively was able to accelerate B‐T healing, as evidenced by better tendon‐to‐bone maturation and increased mechanical property. Recent studies show that peripheral neuropeptides are closely associated with musculoskeletal tissue repair. We furtherly conducted qRT‐PCR and immunofluorescence staining to investigate the temporal‐spatial expression of CGRP, SP and NPY to verify whether they are related to RC healing. Our results show increased expression of CGRP, SP and NPY at healing site under the effect of mechanical stimulation. In conclusion, delayed postoperative exercise with moderate strength appears to accelerate the early phase of B‐T healing, a process that may prove to be linked to increased expression of periphery neuropeptides known to play a role in tissue healing.Keywords
Funding Information
- National Natural Science Foundation of China (81730068)
This publication has 47 references indexed in Scilit:
- Effect of Low-Intensity Pulsed Ultrasound After Autologous Adipose-Derived Stromal Cell Transplantation for Bone-Tendon Healing in a Rabbit ModelThe American Journal of Sports Medicine, 2019
- Unification through disarrayNature Materials, 2017
- Retear Rates After Arthroscopic Single-Row, Double-Row, and Suture Bridge Rotator Cuff Repair at a Minimum of 1 Year of Imaging Follow-up: A Systematic ReviewArthroscopy: The Journal of Arthroscopic & Related Surgery, 2015
- Rotator Cuff Biology and Biomechanics: a Review of Normal and Pathological ConditionsCurrent Rheumatology Reports, 2014
- Assessment and treatment strategies for rotator cuff tearsShoulder & Elbow, 2014
- Effect of Immediate and Delayed High-Strain Loading on Tendon-to-Bone Healing After Anterior Cruciate Ligament ReconstructionJournal of Bone and Joint Surgery, 2014
- Tendon‐to‐bone attachment: From development to maturityBirth Defects Research Part C: Embryo Today: Reviews, 2014
- The Role of Mechanical Loading in Tendon Development, Maintenance, Injury, and RepairJournal of Bone and Joint Surgery, 2013
- The Effect of Postoperative Passive Motion on Rotator Cuff Healing in a Rat ModelJournal of Bone and Joint Surgery, 2009
- The Outcome and Repair Integrity of Completely Arthroscopically Repaired Large and Massive Rotator Cuff TearsThe Journal of Bone & Joint Surgery, 2004