Low-Intensity Pulsed Ultrasound Effect on MIO-M1 Cell Viability: Setup Validation and Standing Waves Analysis
Open Access
- 30 December 2020
- journal article
- research article
- Published by MDPI AG in Applied Sciences
- Vol. 11 (1), 271
- https://doi.org/10.3390/app11010271
Abstract
Low-intensity pulsed ultrasound (LIPUS) has been proposed for novel therapies still under study, where similar parameters and protocols have been used for producing opposite effects that range from increasing cell viability to provoking cell death. Those divergent outcomes make the generalization of expected effects difficult for cell models not yet studied. This paper presents the effect of LIPUS on the viability of the MIO-M1 cell line for two well-established setups and different protocols; the acoustic intensities, duty factors, and treatment duration were varied. Measurements and models for acoustic and thermal analysis are included for proposing a solution to improve the reproducibility of this kind of experiments. Results indicate that MIO-M1 viability is less affected for the cells treated through a dish that is partially immersed in water; in these conditions, the cells neither show detrimental nor proliferative effects at intensities lower than 0.4 W/cm2 at 20% duty factor. However, cell viability was reduced when LIPUS was followed by cell subculturing. Treating the cells through a gel, with the culture dish placed on the transducer, increases cell mortality by the production of standing waves and mixed vibration-acoustical effects. Using the water-based setup with a 1° dish inclination reduces the effects of standing waves.This publication has 31 references indexed in Scilit:
- Prevention of acute kidney injury by low intensity pulsed ultrasound via anti-inflammation and anti-apoptosisScientific Reports, 2020
- Low‑intensity pulsed ultrasound promotes apoptosis and inhibits angiogenesis via p38 signaling‑mediated endoplasmic reticulum stress in human endothelial cellsMolecular Medicine Reports, 2019
- Low Intensity Pulsed Ultrasound (LIPUS) promotes cell motility through vinculin-controlled Rac1 GTPase activityJournal of Cell Science, 2017
- Effects of low-intensity ultrasound on cell proliferation and reproductivityTransactions of Tianjin University, 2016
- Low intensity-pulsed ultrasound induced apoptosis of human hepatocellular carcinoma cells in vitroUltrasonics, 2016
- Enhancement of Neurotrophic Factors in Astrocyte for Neuroprotective Effects in Brain Disorders Using Low-intensity Pulsed Ultrasound StimulationBrain Stimulation, 2015
- In VitroEffects of Low-Intensity Pulsed Ultrasound Stimulation on the Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells for Tooth Tissue EngineeringBioMed Research International, 2013
- Low frequency and intensity ultrasound induces apoptosis of brain glioma in rats mediated by caspase-3, Bcl-2, and survivinBrain Research, 2012
- Effects of low-intensity pulsed ultrasound on osteosarcoma and cancer cellsOncology Reports, 2012
- Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound in a human leukemia cell lineCancer Letters, 2005