Activity of A Novel Succinate Dehydrogenase Inhibitor Fungicide Pydiflumetofen Against Fusarium fujikuroi causing Rice Bakanae Disease
- 1 October 2021
- journal article
- research article
- Published by Scientific Societies in Plant Disease
- Vol. 105 (10), 3208-3217
- https://doi.org/10.1094/pdis-10-20-2274-re
Abstract
New fungicides are tools to manage fungal diseases and overcome emerging resistance in fungal pathogens. In this study, a total of 121 Fusarium fujikuroi isolates were collected from various geographical regions of China and their sensitivity to a novel succinate dehydrogenase inhibitor (SDHI) fungicide ‘pydiflumetofen’ was evaluated. The 50% effective concentration (EC50) value of pydiflumetofen for mycelial growth suppression ranged from 0.0101 to 0.1012 μg/ml and for conidial germination inhibition ranged from 0.0051 to 0.1082 μg/ml. Pydiflumetofen-treated hyphae showed contortion and increased branching, cell membrane permeability, and glycerol content significantly. The result of electron microscope transmission indicated that pydiflumetofen damaged the mycelial cell wall and the cell membrane, and almost broke up the cells, which increased the intracellular plasma leakage. There was no cross-resistance between pydiflumetofen and the widely used fungicides such as carbendazim, prochloraz, and phenamacril. Pydiflumetofen was found safe to seeds and rice seedlings of four rice cultivars, used up to 400 μg/ml. Seed treatment significantly decreased the rate of diseased plants in the greenhouse as well as in field trials in 2017 and 2018. Pydiflumetofen showed superb results against the rice bakanae disease (RBD), when used at 10 or 20 g a.i./100 kg of treated seeds, providing over 90% control efficacy (the maximum control efficacy was up to 97%), which was significantly higher than that of 25% phenamacril (SC) at 10 g or carbendazim at 100 g. Pydiflumetofen is highly effective against F. fujikuroi growth and sporulation as well as RBD in the field.Keywords
Funding Information
- National Key Research and Development Program of China (2016YFD0200806)
- National Natural Science Foundation of China (31872003)
- Natural Science Foundation of Anhui Province (1908085MC95)
- Anhui Provincial “115” Innovation Team, the program for construction of the Key Laboratory of Anhui Academy of Agricultural Sciences (18S1103, 2019YL048, 2020YL073, 2021YL045)
This publication has 30 references indexed in Scilit:
- Effect of phenylpyrrole fungicide fludioxonil on morphological and physiological characteristics of Sclerotinia sclerotiorumPesticide Biochemistry and Physiology, 2013
- A Conserved Homeobox Transcription Factor Htf1 Is Required for Phialide Development and Conidiogenesis in Fusarium SpeciesPLOS ONE, 2012
- Sensitivity of Fusarium verticillioides isolates from rice to a novel cyanoacrylate fungicideCrop Protection, 2012
- Mutagenesis and Functional Studies with Succinate Dehydrogenase Inhibitors in the Wheat Pathogen Mycosphaerella graminicolaPLOS ONE, 2012
- Activity of the Fungicide JS399-19 Against Fusarium Head Blight of Wheat and the Risk of ResistanceAgricultural Sciences in China, 2011
- Progress in understanding molecular mechanisms and evolution of resistance to succinate dehydrogenase inhibiting (SDHI) fungicides in phytopathogenic fungiCrop Protection, 2010
- Sensitivity ofFusarium graminearum to fungicide JS399-19:In vitro determination of baseline sensitivity and the risk of developing fungicide resistancePhytoparasitica, 2008
- Sensitivity of Fusarium moniliforme Isolates to IpconazoleJournal of General Plant Pathology, 2000
- Succinate: quinone oxidoreductases: Variations on a conserved themeBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1997
- Reevaluation of the phenol-sulfuric acid reaction for the estimation of hexoses and pentosesAnalytical Biochemistry, 1989