Circulating CD4+ FoxP3– CXCR5– CXCR3+ PD‐1hi cells are elevated in active rheumatoid arthritis and reflect the severity of the disease
- 6 July 2021
- journal article
- research article
- Published by Wiley in International Journal of Rheumatic Diseases
- Vol. 24 (8), 1032-1039
- https://doi.org/10.1111/1756-185x.14170
Abstract
Objective To examine the expression and clinical significance of circulating CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells in rheumatoid arthritis (RA). Methods CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells in peripheral blood of 35 patients with active RA, 17 with RA in stable remission, and 24 healthy controls were analyzed by flow cytometry. Serum IgG and circulating plasmablast percentages were measured and correlations with CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells were systematically analyzed. Disease Activity Scale 28 (DAS28) scores were also calculated and correlation analysis with CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells was conducted. The levels of CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells were compared before and after disease-modifying anti-rheumatic drug treatment. Cytokine levels in plasma and cytokine secretion in CD4 cells were measured and their correlations with CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells were further analyzed. Results The levels of CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells in the peripheral blood of patients with active RA were significantly increased compared with healthy controls. CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells in patients with active RA were positively correlated with serum IgG and DAS28 scores. CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells were significantly decreased in patients after treatment. Plasma interleukin-10 concentrations and interleukin-10-positive CD4 cell percentages were significantly positively correlated with CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cell levels. Conclusion Circulating CD4+ FoxP3– CXCR5– CXCR3+ PD-1hi cells in patients with active RA are increased and could reflect the severity of the disease, which may play a potential role in the pathogenesis of RA.Keywords
Funding Information
- National Natural Science Foundation of China (81871230)
- Fundamental Research Funds for the Central Universities
This publication has 25 references indexed in Scilit:
- Increased Frequency of Circulating Follicular Helper T Cells in Patients with Rheumatoid ArthritisJournal of Immunology Research, 2012
- Brief Report: Inhibition of interleukin‐6 function corrects Th17/Treg cell imbalance in patients with rheumatoid arthritisArthritis & Rheumatism, 2012
- The development and fate of follicular helper T cells defined by an IL-21 reporter mouseNature Immunology, 2012
- The Pathogenesis of Rheumatoid ArthritisThe New England Journal of Medicine, 2011
- Disturbed Th17/Treg balance in patients with rheumatoid arthritisRheumatology International, 2011
- IL-21 and IL-6 Are Critical for Different Aspects of B Cell Immunity and Redundantly Induce Optimal Follicular Helper CD4 T Cell (Tfh) DifferentiationPLOS ONE, 2011
- The Th17/Treg imbalance and cytokine environment in peripheral blood of patients with rheumatoid arthritisRheumatology International, 2011
- Increased Interleukin 21 (IL-21) and IL-23 Are Associated with Increased Disease Activity and with Radiographic Status in Patients with Early Rheumatoid ArthritisThe Journal of Rheumatology, 2010
- IL-21 acts directly on B cells to regulate Bcl-6 expression and germinal center responsesThe Journal of Experimental Medicine, 2010
- The american rheumatism association 1987 revised criteria for the classification of rheumatoid arthritisArthritis & Rheumatism, 1988