Arabidopsis ACINUS is O-glycosylated and regulates transcription and alternative splicing of regulators of reproductive transitions
Open Access
- 11 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 12 (1), 1-13
- https://doi.org/10.1038/s41467-021-20929-7
Abstract
O-GlcNAc modification plays important roles in metabolic regulation of cellular status. Two homologs of O-GlcNAc transferase, SECRET AGENT (SEC) and SPINDLY (SPY), which have O-GlcNAc and O-fucosyl transferase activities, respectively, are essential in Arabidopsis but have largely unknown cellular targets. Here we show that AtACINUS is O-GlcNAcylated and O-fucosylated and mediates regulation of transcription, alternative splicing (AS), and developmental transitions. Knocking-out both AtACINUS and its distant paralog AtPININ causes severe growth defects including dwarfism, delayed seed germination and flowering, and abscisic acid (ABA) hypersensitivity. Transcriptomic and protein-DNA/RNA interaction analyses demonstrate that AtACINUS represses transcription of the flowering repressor FLC and mediates AS of ABH1 and HAB1, two negative regulators of ABA signaling. Proteomic analyses show AtACINUS’s O-GlcNAcylation, O-fucosylation, and association with splicing factors, chromatin remodelers, and transcriptional regulators. Some AtACINUS/AtPININ-dependent AS events are altered in the sec and spy mutants, demonstrating a function of O-glycosylation in regulating alternative RNA splicing.Funding Information
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (8P41GM103481, 5R01GM066258)
- U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences
This publication has 81 references indexed in Scilit:
- STAR: ultrafast universal RNA-seq alignerBioinformatics, 2012
- The Plant-Specific SR45 Protein Negatively Regulates Glucose and ABA Signaling during Early Seedling Development in ArabidopsisPlant Physiology, 2010
- O-GlcNAc protein modification in plants: Evolution and functionBiochimica et Biophysica Acta (BBA) - General Subjects, 2010
- The hexosamine signaling pathway: O-GlcNAc cycling in feast or famineBiochimica et Biophysica Acta (BBA) - General Subjects, 2010
- Protein Phosphatases 2C Regulate the Activation of the Snf1-Related Kinase OST1 by Abscisic Acid inArabidopsisTHE PLANT CELL ONLINE, 2009
- MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantificationNature Biotechnology, 2008
- Light-Induced Phosphorylation and Degradation of the Negative Regulator PHYTOCHROME-INTERACTING FACTOR1 fromArabidopsisDepend upon Its Direct Physical Interactions with Photoactivated PhytochromesTHE PLANT CELL ONLINE, 2008
- Acinus-S′ Represses Retinoic Acid Receptor (RAR)-Regulated Gene Expression through Interaction with the B Domains of RARsMolecular and Cellular Biology, 2008
- PrDOS: prediction of disordered protein regions from amino acid sequenceNucleic Acids Research, 2007
- The structure and function of small nucleolar ribonucleoproteinsNucleic Acids Research, 2007