Changes in Methane Emission and Community Composition of Methane-Cycling Microorganisms Along an Elevation Gradient in the Dongting Lake Floodplain, China

Abstract

Methane (CH₄) emission and environmental controls of CH₄-cycling microorganisms are unclear in inland floodplains. Here, we examined soil CH₄ emissions and the community composition of CH₄-cycling microorganisms under three vegetation types—mudflat (MF, no vegetation cover), Carex meadow (CM, mainly Carex brevicuspis), and reed land (RL, mainly Miscanthus sacchariflorus)—from water-adjacent areas to higher-elevation land in the Dongting Lake floodplain, China. The results showed that CH₄ emission is the highest in CM, while significant absorption was observed in the RL site. The abundance ratio of methanogen/methanotroph was the highest in CM, intermediate in MF, and lowest in RL. The Methanosarcinaceae family represented the dominant methanogens in the three sampling sites (41.32–75.25%). The genus Methylocystis (60.85%, type II methanotrophs) was dominant in CM, while Methylobacter and Methylosarcina (type I methanotrophs) were the dominant genera in MF (51.00%) and RL (50.24%), respectively. Structural equation model analysis showed that methanogen and methanotroph abundance were affected by water table depth, soil water content, and pH indirectly through soil organic content, total nitrogen, microbial biomass carbon, and microbial biomass nitrogen. These results indicated that the Dongting Lake floodplain may change from a CH₄ source to a CH₄ sink with vegetation succession with an increase in elevation, and the methanogen/methanotroph ratio can be used as a proxy for CH₄ emission in wetland soils. The continuous increase in reed area combined with the decrease in Carex meadow may mitigate CH₄ emission and enhance the CH₄ sink function during the non-flood season in the Dongting Lake floodplain.