Thematic study is an area in any literary study that cannot be easily overlooked. It is an area that cuts across the three major genres of literature. This paper examines the literary text Ajo Obi which is written by I.G. Nwaozuzu in form of drama with the view of studying the theme of infidelity as portrayed in the text. This work aims at showing the ambiguity of the claim of innocence by the accused in the text. It also brings to light how cultural divergence brings about different interpretations of an action. The data for this study are drawn from intensive study of the literary text Ajo Obi. Following a qualitative research paradigm the study adopts reader response approach in its analysis. Findings show that there is a kind of uncertainty in the claim of innocence by the accused in the text. Again cultural divergence is also found to bring about differences in interpretation of an action.