Genome-Wide Identification of the B-BOX Genes that Respond to Multiple Ripening Related Signals in Sweet Cherry Fruit
Open Access
- 5 February 2021
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 22 (4), 1622
- https://doi.org/10.3390/ijms22041622
Abstract
B-BOX proteins are zinc finger transcription factors that play important roles in plant growth, development, and abiotic stress responses. In this study, we identified 15 PavBBX genes in the genome database of sweet cherry. We systematically analyzed the gene structures, clustering characteristics, and expression patterns of these genes during fruit development and in response to light and various hormones. The PavBBX genes were divided into five subgroups. The promoter regions of the PavBBX genes contain cis-acting elements related to plant development, hormones, and stress. qRT-PCR revealed five upregulated and eight downregulated PavBBX genes during fruit development. In addition, PavBBX6, PavBBX9, and PavBBX11 were upregulated in response to light induction. We also found that ABA, BR, and GA3 contents significantly increased in response to light induction. Furthermore, the expression of several PavBBX genes was highly correlated with the expression of anthocyanin biosynthesis genes, light-responsive genes, and genes that function in multiple hormone signaling pathways. Some PavBBX genes were strongly induced by ABA, GA, and BR treatment. Notably, PavBBX6 and PavBBX9 responded to all three hormones. Taken together, BBX proteins likely play major roles in regulating anthocyanin biosynthesis in sweet cherry fruit by integrating light, ABA, GA, and BR signaling pathways.Funding Information
- National Natural Science Foundation of China (31672119; 31972390)
- National Key Research and Development Program of China (2019YFD1000102-02)
- Beijing Science and Technology Innovation and Service Capacity in Top Subjects (CEFF-PXM2019_014207_000032)
This publication has 60 references indexed in Scilit:
- Genome-wide identification and analysis of B-BOX gene family in grapevine reveal its potential functions in berry developmentBMC Plant Biology, 2020
- BBX21, an Arabidopsis B-box protein, directly activates HY5 and is targeted by COP1 for 26S proteasome-mediated degradationProceedings of the National Academy of Sciences of the United States of America, 2016
- The BBX family of plant transcription factorsTrends in Plant Science, 2014
- A Zinc Finger Protein Regulates Flowering Time and Abiotic Stress Tolerance in Chrysanthemum by Modulating Gibberellin BiosynthesisTHE PLANT CELL ONLINE, 2014
- TheArabidopsisB-Box Zinc Finger FamilyTHE PLANT CELL ONLINE, 2009
- The Evolution of CONSTANS-Like Gene Families in Barley, Rice, and ArabidopsisPlant Physiology, 2003
- Functional importance of conserved domains in the flowering‐time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plantsThe Plant Journal, 2001
- Arabidopsis Transcription Factors: Genome-Wide Comparative Analysis Among EukaryotesScience, 2000
- Zinc-finger transcription factors in plantsCellular and Molecular Life Sciences, 1998
- The CONSTANS gene of arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factorsCell, 1995