There are a number of springs on the territory of Lviv, water from which is used by the city residents. It should be noted that natural resources cannot be systematically used by the population for drinking purposes, as they are not sources of centralized water supply and, accordingly, do not have service personnel. No sanitary protection zones have been established for such sources, within which economic activities are prohibited. In this regard, the quality of water from such natural sources has unstable indicators, which depend on many factors of man-made and natural factors (sanitary-chemical and sanitary-microbiological indicators can sometimes fluctuate within one week), which makes it unfit for human consumption and can lead to acute intestinal infections of bacterial and viral etiology, a number of infectious diseases. The article considers the current ecological, microbiological state of natural sources of the city of Lviv. The necessity, and hence the relevance of the analysis and detailed microbiological assessment of water quality of springs in the city of Lviv, namely: springs № 1 in the park "Zalizna Voda" (Lviv, Ternopilska Street); springs № 2 in the park "Zalizna Voda" (Lviv, Myshuhy Street); springs № 3 in Stryiskyi Park (Lviv, Stryiska Street). A sociological survey of city residents on the use and consumption of water from the studied sources was conducted. The results of the analysis were compared with the list of maximum concentration limits of harmful substances in water bodies in accordance with the order № 400 from 12.05.2010 on approval of State sanitary norms and rules “Hygienic requirements for drinking water intended for human consumption” (DSanPiN 2.2.4-171-10).