Recent advances in nanomaterial-enhanced enzyme-linked immunosorbent assays
- 21 June 2020
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 145 (12), 4069-4078
- https://doi.org/10.1039/d0an00597e
Abstract
Despite serving as a gold standard for protein analysis, the classic enzyme-linked immunosorbent assay (ELISA) is currently challenged by the ever-increasing needs of sensitivity and simplicity. Towards the ongoing needs, recent advances in nanomaterials have offered numerous promising tools for enhancing the performance and broadening the applicability of ELISA. In this review, we highlight nanomaterial-enabled strategies that drastically improve the assay performance without significantly altering the classic ELISA format. Particular attention will be focused on the functional roles of nanomaterials as novel readout systems in ELISA, including those serving as substrate-alternatives, enzyme-alternatives, and non-enzymatic signal amplifiers.Funding Information
- Fundamental Research Funds for the Central Universities
- Natural Sciences and Engineering Research Council of Canada (RGPIN05240)
- Ontario Ministry of Research, Innovation and Science
This publication has 111 references indexed in Scilit:
- Dynamic DNA Assemblies Mediated by Binding-Induced DNA Strand DisplacementJournal of the American Chemical Society, 2013
- A Molecular Translator that Acts by Binding‐Induced DNA Strand Displacement for a Homogeneous Protein AssayAngewandte Chemie, 2012
- Immuno-Hybridization Chain Reaction for Enhancing Detection of Individual Cytokine-Secreting Human Peripheral Mononuclear CellsAnalytical Chemistry, 2011
- Gain-of-function GPIb ELISA assay for VWF activity in the Zimmerman Program for the Molecular and Clinical Biology of VWDBlood, 2011
- Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrationsNature Biotechnology, 2010
- Nanoparticle-based bio-barcode assay redefines “undetectable” PSA and biochemical recurrence after radical prostatectomyProceedings of the National Academy of Sciences of the United States of America, 2009
- Oxidase‐Like Activity of Polymer‐Coated Cerium Oxide NanoparticlesAngewandte Chemie-International Edition, 2009
- Determination of food authenticity by enzyme-linked immunosorbent assay (ELISA)Food Control, 2008
- Protein detection using proximity-dependent DNA ligation assaysNature Biotechnology, 2002
- Nucleic acid junctions and latticesJournal of Theoretical Biology, 1982