3D super-resolution deep-tissue imaging in living mice
Open Access
- 25 March 2021
- journal article
- research article
- Published by Optica Publishing Group in Optica
- Vol. 8 (4), 442-450
- https://doi.org/10.1364/optica.416841
Abstract
Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of two-photon excitation, adaptive optics, red-emitting organic dyes, and a long-working-distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 mu m deep in fixed mouse brain tissue and 76 mu m deep in the brain of a living mouse. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.Funding Information
- Yale’s Integrated Graduate Program in Physical and Engineering Biology
- Wellcome Trust (203285/B/16/Z, 203285/C/16/Z)
- European Research Council (AdOMIS 695140)
- G. Harold and Leila Y. Mathers Foundation
- National Institutes of Health (1R01NS089734, P30-DK45735, U01 DA047734)
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