Sodium Copper Chlorophyllin Is Highly Effective against Enterovirus (EV) A71 Infection by Blocking Its Entry into the Host Cell
- 1 April 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Infectious Diseases
- Vol. 6 (5), 882-890
- https://doi.org/10.1021/acsinfecdis.0c00096
Abstract
Human enteroviruses (HEVs) pose an ongoing threat to global public health. Particularly, enterovirus-A71 (EV-A71), the main pathogen causing hand-foot-and-mouth disease (HFMD), has caused ongoing outbreaks globally in recent years associated with severe neurological manifestations and several deaths cases. Currently, no effective antivirals are available for the prevention or treatment of EV-A71 infection. In this study, we found that sodium copper chlorophyllin (CHL), a health food additive and an over-the-counter medicine for anti-cancer or reducing the odor of urine or feces, exhibited potent inhibitory activity against infection by divergent EV-A71 and coxsackievirus-A16 (CV-A16) isolates at low micromolar concentration with excellent safety. Their antiviral activities were confirmed by a colorimetric viral infection and qRT-PCR assays. A series of mechanistic studies showed that CHL did not target the host cell, but block the entry of EV-A71 and CV-A16 into the host cell at the post-attachment stage. In the mouse model, CHL could significantly reduce the viral titer in the lungs and muscles. Since CHL has used in clinics for many years with excellent safety, it has potential to be further developed into a prophylactic or therapeutic to prevent or treat HFMD caused by EV-A71 or CV-A16 infection.Keywords
Funding Information
- Ministry of Science and Technology of the People's Republic of China (2018ZX10301403)
- China Postdoctoral Science Foundation (2018M640341, 2019T120302)
- National Natural Science Foundation of China (81630090, 81661128041, 81672019, 81701998, 81703571, 81822045)
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