Endopolyploidy Variation in Wild Barley Seeds across Environmental Gradients in Israel
Open Access
- 10 May 2021
- Vol. 12 (5), 711
- https://doi.org/10.3390/genes12050711
Abstract
Wild barley is abundant, occupying large diversity of sites, ranging from the northern mesic Mediterranean meadows to the southern xeric deserts in Israel. This is also reflected in its wide phenotypic heterogeneity. We investigated the dynamics of DNA content changes in seed tissues in ten wild barley accessions that originated from an environmental gradient in Israel. The flow cytometric measurements were done from the time shortly after pollination up to the dry seeds. We show variation in mitotic cell cycle and endoreduplication dynamics in both diploid seed tissues (represented by seed maternal tissues and embryo) and in the triploid endosperm. We found that wild barley accessions collected at harsher xeric environmental conditions produce higher proportion of endoreduplicated nuclei in endosperm tissues. Also, a comparison of wild and cultivated barley strains revealed a higher endopolyploidy level in the endosperm of wild barley, that is accompanied by temporal changes in the timing of the major developmental phases. In summary, we present a new direction of research focusing on connecting spatiotemporal patterns of endoreduplication in barley seeds and possibly buffering for stress conditions.Keywords
Funding Information
- Grantová Agentura České Republiky (18-12197S, 21-02929S, 20-25845Y)
- European Regional Development Fund (CZ.02.1.01/0.0/0.0/16_019/0000827)
This publication has 58 references indexed in Scilit:
- Endoreduplication intensity as a marker of seed developmental stage in the FabaceaeCytometry Part A, 2012
- Combined linkage and association mapping reveals CYCD5;1 as a quantitative trait gene for endoreduplication in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2012
- Elucidating the functional role of endoreduplication in tomato fruit developmentAnnals of Botany, 2011
- Laser microdissection of Arabidopsis cells at the powdery mildew infection site reveals site-specific processes and regulatorsProceedings of the National Academy of Sciences of the United States of America, 2009
- The Development of Endosperm in GrassesPlant Physiology, 2009
- Barley Grain Maturation and Germination: Metabolic Pathway and Regulatory Network Commonalities and Differences Highlighted by New MapMan/PageMan Profiling ToolsPlant Physiology, 2008
- ENDOSPERM DEVELOPMENT: Cellularization and Cell Fate SpecificationAnnual Review of Plant Physiology and Plant Molecular Biology, 2001
- Genetic Resources for Salt Tolerance in the Wild Progenitors of Wheat (Triticum dicoccoides) and Barley (Hordeum spontaneum) in IsraelPlant Breeding, 1993
- Genetic diversity and environmental associations of wild emmer wheat, in TurkeyHeredity, 1988
- Genetic diversity and environmental associations of wild barley,Hordeum spontaneum (Poaceae), in IranÖsterreichische botanische Zeitschrift, 1986