Phytochemical Screening and In-vitro Antioxidant and Antiproliferative Activity of Aqueous Leaf Extract of Ximtenia americana against Non- Small Cell Lung Cancer

Abstract

Objective of present work to screening of phytochemicals and in vitro antioxidant and antiproliferative activity of aqueous leaf extract of Ximenia americana against non-small cell lung cancer. Herbal extraction was done by Soxhlet extraction method with distilled water. Phytochemical analysis was done using different standard biochemical tests. Antioxidant potential of plant extract was analyzed by Hydrogen peroxide assay and antiproliferative activity by using in-vitro MTT assay against non-small lung cancer cell lines (A549 &NCI-H460). Total flavonoids content of aqueous extract was also determined to assess their corresponding effect on antioxidant capacity of plant. Phytochemical analysis showed that each solvent extracts contained broad spectrum of secondary metabolites, phenolic compounds, flavonoids, tannins and glycosides and also aqueous extract exhibited the highest flavonoids content and the significant antioxidant capacity based on the test performed. In case antiproliferative assay aqueous extract shown very good cytotoxic activity by inhibiting the growth in both cell lines. IC50 value found to be 229.20 μg and 338.30 μg for A549 and NCI-H460 respectively. The present study revealed that aqueous leaf extract of X. americana leaves contain broad spectrum of bioactive compounds. Results confirm that aqueous extract exhibited high antioxidant activity and flavonoids content. In MTT assay it shown significant antiproliferative activity against both non-small cell lung cancer cell lines. Further study requires purification, Characterization and structural elucidation of flavoind compounds in aqueous extract and its pharmacological studies in animal models i.e. In-vivo study which that may help in the development of new novel drug.