The Effect of Hibiscus sabdariffa Extracts on Pseudomonas aeruginosa Isolated from Wounds and Pus

Abstract

The study aimed to evaluate the efficacy of Hibiscus sabdariffa extracts of aqueous and alcoholic extracts on Pseudomonas aeruginosa using agar well diffusion method. The study enrolled 100 wound and pus patients. The results showed that 44 isolates were Pseudomonas aeruginosa that equivalent to 44% total isolates. The Hibiscus sabdariffa extract concentrations used are (10, 25, 50,75 and 100 mg/ml). The bacterial isolates identified after been growing on blood agar and purified on MacConkey Agar using many biochemical tests. The bacterial identification was confirmed using Vitek-2 compact system technique. The results showed that the aqueous extracts were more active than alcoholic extract and no inhibition at the concentrations (10, 25 and 50 mg/ml) against Pseudomonas aeruginosa for aqueous and alcoholic extracts, the inhibition zones were (11 and 13) mm at the concentrations (75 and 100) mg/ml respectively for aqueous extract, and (10 and 11) mm at the concentrations (75 and 100) mg/ml respectively for alcoholic. Sensitivity test was conducted for Pseudomonas aeruginosa using Vitec compact test. FTIR and GC/MS tests conducted for the plant extracts and the results detailed in the study.