Suitability of a Cellulose-Based Nanomaterial for the Remediation of Heavy Metal Contaminated Freshwaters: A Case-Study Showing the Recovery of Cadmium Induced DNA Integrity Loss, Cell Proliferation Increase, Nuclear Morphology and Chromosomal Alterations on Dreissena polymorpha
Open Access
- 14 September 2020
- journal article
- research article
- Published by MDPI AG in Nanomaterials
- Vol. 10 (9), 1837
- https://doi.org/10.3390/nano10091837
Abstract
The contamination of freshwaters by heavy metals represents a great problem, posing a threat for human and environmental health. Cadmium is classified as carcinogen to humans and its mechanism of carcinogenicity includes genotoxic events. In this study a recently developed eco-friendly cellulose-based nanosponge (CNS) was investigated as a candidate in freshwater nano-remediation process. For this purpose, CdCl2 (0.05 mg L−1) contaminated artificial freshwater (AFW) was treated with CNS (1.25 g L−1 for 2 h), and cellular responses were analyzed before and after CNS treatment in Dreissena polymorpha hemocytes. A control group (AFW) and a negative control group (CNS in AFW) were also tested. DNA primary damage was evaluated by Comet assay while chromosomal damage and cell proliferation were assessed by Cytome assay. AFW exposed to CNS did not cause any genotoxic effect in zebra mussel hemocytes. Moreover, DNA damage and cell proliferation induced by Cd(II) turned down to control level after 2 days when CNS were used. A reduction of Cd(II)-induced micronuclei and nuclear abnormalities was also observed. CNS was thus found to be a safe and effective candidate in cadmium remediation process being efficient in metal sequestering, restoring cellular damage exerted by Cd(II) exposure, without altering cellular physiological activity.Keywords
This publication has 66 references indexed in Scilit:
- Multiple roles of cadmium in cell death and survivalChemico-Biological Interactions, 2010
- Antioxidant, genotoxic and lysosomal biomarkers in the freshwater bivalve (Unio pictorum) transplanted in a metal polluted river basinAquatic Toxicology, 2010
- Cadmium stress: an oxidative challengeBioMetals, 2010
- Nanotechnology and in Situ Remediation: A Review of the Benefits and Potential RisksEnvironmental Health Perspectives, 2009
- Role of oxidative stress in cadmium toxicity and carcinogenesisToxicology and Applied Pharmacology, 2009
- Inducibility of metallothionein biosynthesis in the whole soft tissue of zebra mussels Dreissena polymorpha exposed to cadmium, copper, and pentachlorophenolEnvironmental Toxicology and Water Quality, 2009
- Elevated Cadmium Concentrations in Potato Tubers Due to Irrigation with River Water Contaminated by Mining in Potosí, BoliviaJournal of Environmental Quality, 2007
- Biomarkers in zebra mussels (Dreissena polymorpha) for the assessment and monitoring of water quality of the St Lawrence River (Canada)Aquatic Toxicology, 2000
- Sublethal effects of cadmium on physiological responses in the pocketbook mussel, Lampsilis ventricosaEnvironmental Toxicology and Chemistry, 1992
- A simple technique for quantitation of low levels of DNA damage in individual cellsExperimental Cell Research, 1988