The biGBac system presented by Weissmann et al. (Weissmann et al, 2016 and Weissmann et al, 2018) is a modular insect cell expression system designed for expression of multi-subunit complexes. The system is very powerful, allowing up to 25 genes to be assembled into one baculo-virus expression vector, but does require a reasonable amount of cloning experience in order to attain clones in the 6 day time frame stated in the paper. This guide was written to provide a detailed explanation how the system is designed, how it can be further exploited and provide in-depth practical information that even a novice cloner can follow.