Introduction to Single-Cell DNA Methylation Profiling Methods
Open Access
- 10 July 2021
- journal article
- review article
- Published by MDPI AG in Biomolecules
- Vol. 11 (7), 1013
- https://doi.org/10.3390/biom11071013
Abstract
DNA methylation is an epigenetic mechanism that is related to mammalian cellular differentiation, gene expression regulation, and disease. In several studies, DNA methylation has been identified as an effective marker to identify differences between cells. In this review, we introduce single-cell DNA-methylation profiling methods, including experimental strategies and approaches to computational data analysis. Furthermore, the blind spots of the basic analysis and recent alternatives are briefly described. In addition, we introduce well-known applications and discuss future development.Keywords
Funding Information
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean Government (MSIT; 2016M3A9B6948494, MSIT; 2018R1A2A1A05079172, MSIT; 2021R1A2C2008490, HI18C2282, HI14C1277)
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